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Literature summary extracted from

  • Schloss, J.V.; VanDyk, D.E.; Vasta, J.F.; Kutny, R.M.
    Purification and properties of Salmonella typhimurium acetolactate synthase isozyme II from Escherichia coli HB101/pDU9 (1985), Biochemistry, 24, 4952-4959.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ required Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.6 Mg2+ Km: 0.022 mM Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 9800
-
 2 * 9800 + 2 * 59000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.6 59000
-
 2 * 9800 + 2 * 59000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.6 140000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Salmonella enterica subsp. enterica serovar Typhimurium
-
 isoenzyme II, recombinantly produced in Escherichia coli HB101/pDU9
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.2.1.6 oxygen-dependent inactivation of the enzyme complexed with thiamine diphosphate, FAD and magnesium acetolactate, no inactivation of the enzyme-FAD complex Salmonella enterica subsp. enterica serovar Typhimurium

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6 isoenzyme II, recombinantly produced Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 25.3
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.2.1.6 -80°C, concentrated enzyme solution is indefinitely stable Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-acetolactate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 tetramer 2 * 9800 + 2 * 59000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD contains bound FAD, 0.88 mol per mol of protomer of 69000 Da Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.6 thiamine diphosphate required as cofactor Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.6 thiamine diphosphate Km: 0.0015 mM Salmonella enterica subsp. enterica serovar Typhimurium