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Literature summary extracted from

  • Singh, B.K.; Stidham, M.A.; Shaner, D.L.
    Assay of acetohydroxyacid synthase (1988), Anal. Biochem., 171, 173-179.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 5
-
 pyruvate
-
 Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ saturated at 1 mM MgCl2 Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.2.1.6 cell suspension culture
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 additional information
-
 spectrophotometric assay involving an indirect detection of the product acetolactate Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Zea mays 2-acetolactate + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.6 46 50
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 6 7
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.2.1.6 5.5 8
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD 0.005 mM, 35-40% enhancement of activity Zea mays
2.2.1.6 thiamine diphosphate required as cofactor Zea mays
2.2.1.6 thiamine diphosphate saturated at 0.5 mM thiamine diphosphate Zea mays