Literature summary extracted from
Madhusudanan Nair, P.; Vaidyanathan, C.S.
An indole oxidase isolated from the leaves of Tecoma stans (1964), Biochim. Biophys. Acta, 81, 496-506.
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.13.11.17 |
8-hydroxyquinoline |
0.5 mM, 40% inhibition |
Tecoma stans |
|
1.13.11.17 |
Atebrin |
0.5 mM, 80% inhibition |
Tecoma stans |
|
1.13.11.17 |
Cys |
Cu2+ can completely reverse inhibition |
Tecoma stans |
|
1.13.11.17 |
diethyldithiocarbamate |
0.5 mM, 60% inhibition, Cu2+ can completely reverse inhibition |
Tecoma stans |
|
1.13.11.17 |
Fe2+ |
0.5 mM FeSO4, 35% inhibition |
Tecoma stans |
|
1.13.11.17 |
Fe3+ |
0.5 mM Fe2(SO4)3, 50% inhibition |
Tecoma stans |
|
1.13.11.17 |
GSH |
Cu2+ can completely reverse inhibition |
Tecoma stans |
|
1.13.11.17 |
Hg2+ |
0.5 mM HgCl2, 30% inhibition |
Tecoma stans |
|
1.13.11.17 |
NEM |
slight |
Tecoma stans |
|
1.13.11.17 |
PCMB |
0.5 mM, 30% inhibition, Cu2+ can completely reverse inhibition |
Tecoma stans |
|
1.13.11.17 |
Salicylaldoxime |
0.5 mM, 62% inhibition |
Tecoma stans |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.13.11.17 |
Cu2+ |
absolute requirement for maximal activity |
Tecoma stans |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.11.17 |
Tecoma stans |
- |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.11.17 |
indole + O2 |
- |
Tecoma stans |
anthranilic acid |
final product is anthranil, formylaminobenzaldehyde and o-aminobenzaldehyde are intermediates, 3 atoms of oxygen are consumed in the formation of anthranil from one molecule of indole |
? |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.13.11.17 |
5 |
- |
- |
Tecoma stans |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
1.13.11.17 |
3.8 |
6.2 |
pH 3.8: about 80% of maximal activity, pH 5.8: about 65% of maximal activity, pH 6.2: about 30% of maximal activity |
Tecoma stans |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.13.11.17 |
FAD |
absolute requirement for maximal activity |
Tecoma stans |
|