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Literature summary extracted from

  • Madhusudanan Nair, P.; Vaidyanathan, C.S.
    An indole oxidase isolated from the leaves of Tecoma stans (1964), Biochim. Biophys. Acta, 81, 496-506.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.17 8-hydroxyquinoline 0.5 mM, 40% inhibition Tecoma stans
1.13.11.17 Atebrin 0.5 mM, 80% inhibition Tecoma stans
1.13.11.17 Cys Cu2+ can completely reverse inhibition Tecoma stans
1.13.11.17 diethyldithiocarbamate 0.5 mM, 60% inhibition, Cu2+ can completely reverse inhibition Tecoma stans
1.13.11.17 Fe2+ 0.5 mM FeSO4, 35% inhibition Tecoma stans
1.13.11.17 Fe3+ 0.5 mM Fe2(SO4)3, 50% inhibition Tecoma stans
1.13.11.17 GSH Cu2+ can completely reverse inhibition Tecoma stans
1.13.11.17 Hg2+ 0.5 mM HgCl2, 30% inhibition Tecoma stans
1.13.11.17 NEM slight Tecoma stans
1.13.11.17 PCMB 0.5 mM, 30% inhibition, Cu2+ can completely reverse inhibition Tecoma stans
1.13.11.17 Salicylaldoxime 0.5 mM, 62% inhibition Tecoma stans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.17 Cu2+ absolute requirement for maximal activity Tecoma stans

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.17 Tecoma stans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.17 indole + O2
-
Tecoma stans anthranilic acid final product is anthranil, formylaminobenzaldehyde and o-aminobenzaldehyde are intermediates, 3 atoms of oxygen are consumed in the formation of anthranil from one molecule of indole ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.17 5
-
-
Tecoma stans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.17 3.8 6.2 pH 3.8: about 80% of maximal activity, pH 5.8: about 65% of maximal activity, pH 6.2: about 30% of maximal activity Tecoma stans

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.17 FAD absolute requirement for maximal activity Tecoma stans