Literature summary extracted from

Rieder, R.; Cammack, R.; Hall, D.O.
Purification and properties of the soluble hydrogenase from Desulfovibrio desulfuricans (strain Norway 4) (1984), Eur. J. Biochem., 145, 637-643.

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.12.2.1	membrane	bound to	Desulfovibrio desulfuricans	16020	-
1.12.2.1	soluble	-	Desulfovibrio desulfuricans	-	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.12.2.1	Iron	4.8 iron atoms per molecule	Desulfovibrio desulfuricans
1.12.2.1	Nickel	0.45 nickel atoms per molecule	Desulfovibrio desulfuricans
1.12.2.1	selenium	0.45 selenium atoms per molecule	Desulfovibrio desulfuricans

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.12.2.1	27100	-	1 * 27100 + 1 * 59800, membrane-bound enzyme, SDS-PAGE	Desulfovibrio desulfuricans
1.12.2.1	28600	-	1 * 28600 + 1 * 56400, soluble enzyme, SDS-PAGE	Desulfovibrio desulfuricans
1.12.2.1	56400	-	1 * 28600 + 1 * 56400, soluble enzyme, SDS-PAGE	Desulfovibrio desulfuricans
1.12.2.1	59800	-	1 * 27100 + 1 * 59800, membrane-bound enzyme, SDS-PAGE	Desulfovibrio desulfuricans
1.12.2.1	61000	-	membrane-bound enzyme, gel filtration	Desulfovibrio desulfuricans
1.12.2.1	67000	-	soluble enzyme, gel filtration	Desulfovibrio desulfuricans

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.12.2.1	Desulfovibrio desulfuricans	-	Norway 4	-
1.12.2.1	Desulfovibrio desulfuricans Norway 4	-	Norway 4	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.12.2.1	soluble enzyme	Desulfovibrio desulfuricans

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.12.2.1	250	-	-	Desulfovibrio desulfuricans

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.12.2.1	H2 + ferricytochrome c3	-	Desulfovibrio desulfuricans	H+ + ferrocytochrome c3	-	?
1.12.2.1	H2 + ferricytochrome c3	-	Desulfovibrio desulfuricans Norway 4	H+ + ferrocytochrome c3	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.12.2.1	dimer	1 * 28600 + 1 * 56400, soluble enzyme, SDS-PAGE	Desulfovibrio desulfuricans
1.12.2.1	dimer	1 * 27100 + 1 * 59800, membrane-bound enzyme, SDS-PAGE	Desulfovibrio desulfuricans

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.12.2.1	additional information	-	additional information	-	Desulfovibrio desulfuricans

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.12.2.1	cytochrome c3	-	Desulfovibrio desulfuricans

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Release 2023.1 (January 2023)