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Literature summary extracted from

  • Yagi, T.; Honya, M.; Tamiya, N.
    Purification and properties of hydrogenases of different origins (1968), Biochim. Biophys. Acta, 153, 699-705.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.2.1 CO
-
Desulfovibrio desulfuricans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.2.1 0.075
-
ferrocytochrome c3 H2-evolution reaction Desulfovibrio desulfuricans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.2.1 60000
-
gel filtration Desulfovibrio desulfuricans

Organism

EC Number Organism UniProt Comment Textmining
1.12.2.1 Desulfovibrio desulfuricans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.2.1
-
Desulfovibrio desulfuricans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.2.1 H+ + reduced methyl viologen
-
Desulfovibrio desulfuricans H2 + methyl viologen
-
?
1.12.2.1 H2 + ferricytochrome c3
-
Desulfovibrio desulfuricans H+ + ferrocytochrome c3
-
r
1.12.2.1 additional information enzyme catalyzes production of H2 from Na2S2O4 in presence of cytochrome c3 Desulfovibrio desulfuricans ?
-
?
1.12.2.1 additional information enzyme can catalyze H-2H exchange in absence of added electron carriers Desulfovibrio desulfuricans ?
-
?
1.12.2.1 additional information no reduction of ferredoxin, methylene blue or hexacyanoferrate(II) by H2 Desulfovibrio desulfuricans ?
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.2.1 50
-
10 min, H2 evolution in presence of methyl viologen, no decrease in activity Desulfovibrio desulfuricans
1.12.2.1 60
-
10 min, H2 evolution in presence of methyl viologen, 30% loss of activity Desulfovibrio desulfuricans
1.12.2.1 70
-
10 min, H2 evolution in presence of methyl viologen, 37% loss of activity Desulfovibrio desulfuricans
1.12.2.1 80
-
10 min, H2 evolution in presence of methyl viologen, 69% loss of activity Desulfovibrio desulfuricans
1.12.2.1 90
-
10 min, H2 evolution in presence of methyl viologen, 99% loss of activity Desulfovibrio desulfuricans
1.12.2.1 100
-
10 min, H2 evolution in presence of methyl viologen, complete loss of activity Desulfovibrio desulfuricans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.2.1 5 6 cytochrome c3-dependent H2 evolution Desulfovibrio desulfuricans
1.12.2.1 6 7 methyl viologen-dependent H2 evolution Desulfovibrio desulfuricans
1.12.2.1 6 8 H-2H exchange Desulfovibrio desulfuricans
1.12.2.1 8 9 cytochrome c3 reduction by H2 Desulfovibrio desulfuricans

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.2.1 cytochrome c3
-
Desulfovibrio desulfuricans