EC Number | General Stability | Organism |
---|---|---|
1.13.11.52 | guanidine hydrochloride, 30°C, 30 min, about 15% loss of activity at 0.5 M, about 30% loss of activity at 1 mM, about 60% loss of activity at 2 M, complete loss of activity at 3 mM | Brevibacillus brevis |
1.13.11.52 | urea, 30°C, 30 min, stable at 2.5 M, about 30% loss of activity at 5.0 M, about 60% loss of activity at 7.5 M | Brevibacillus brevis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.52 | CN- | 0.4 mM, 86% inhibition | Brevibacillus brevis | |
1.13.11.52 | CO | 20% with 80% air, 78% inhibition | Brevibacillus brevis | |
1.13.11.52 | D-Trp | no inhibition | Brevibacillus brevis | |
1.13.11.52 | DL-5-hydroxytryptophan | 1 mM, 55% inhibition | Brevibacillus brevis | |
1.13.11.52 | indole | 1 mM, 44% inhibition | Brevibacillus brevis | |
1.13.11.52 | Indolepropionic acid | 1 mM, 31% inhibition | Brevibacillus brevis | |
1.13.11.52 | K3Fe(CN)6 | 1 mM, complete inhibition | Brevibacillus brevis | |
1.13.11.52 | N3- | 2 mM, 22% inhibition | Brevibacillus brevis | |
1.13.11.52 | NH2OH | 2 mM, 54% inhibition | Brevibacillus brevis | |
1.13.11.52 | tryptamine | 1 mM, 23% inhibition | Brevibacillus brevis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.52 | 0.044 | - |
O2 | - |
Brevibacillus brevis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.52 | 27000 | - |
4 * 27000, SDS-PAGE | Brevibacillus brevis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.52 | Brevibacillus brevis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.52 | - |
Brevibacillus brevis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.52 | 3.85 | - |
- |
Brevibacillus brevis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.52 | DL-5-fluoro-methyltryptophan + O2 | 35% of the activity with L-Trp | Brevibacillus brevis | ? | - |
? | |
1.13.11.52 | DL-5-methyltryptophan + O2 | 90% of the activity with L-Trp | Brevibacillus brevis | ? | - |
? | |
1.13.11.52 | DL-6-fluorotryptophan + O2 | 90% of the activity with L-Trp | Brevibacillus brevis | ? | - |
? | |
1.13.11.52 | DL-6-methyltryptophan | 98% of the activity with L-Trp | Brevibacillus brevis | ? | - |
? | |
1.13.11.52 | L-Trp + O2 | - |
Brevibacillus brevis | L-formylkynurenine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.52 | tetramer | 4 * 27000, SDS-PAGE | Brevibacillus brevis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.52 | 55 | - |
- |
Brevibacillus brevis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.52 | additional information | - |
L-Trp stabilizes against heat inactivation | Brevibacillus brevis |
1.13.11.52 | 70 | - |
30 min, stable in presence of 5 mM L-Trp | Brevibacillus brevis |
1.13.11.52 | 75 | - |
30 min, about 5% loss of activity in presence of 5 mM L-Trp | Brevibacillus brevis |
1.13.11.52 | 80 | - |
30 min, about 35% loss of activity in presence of 5 mM L-Trp | Brevibacillus brevis |
1.13.11.52 | 85 | - |
30 min, complete loss of activity in presence of 5 mM L-Trp | Brevibacillus brevis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.52 | 7.5 | - |
- |
Brevibacillus brevis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.52 | heme | contains 1 mol or more of heme per mol of enzyme | Brevibacillus brevis |