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Literature summary extracted from

  • Lindstedt, S.; Odelhoeg, B.; Rundgren, M.
    Purification and some properties of 4-hydroxyphenylpyruvate dioxygenase from Pseudomonas sp. P. J. 874 (1977), Biochemistry, 16, 3369-3377.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.27 2,6-dichlorophenol indophenol competitive activator Pseudomonas sp.
1.13.11.27 ascorbate an optimized combination of ascorbate, 50 mM, catalase, 2.4 g/l, and iron, 0.05 mM, activates more than a combination of 2,6-dichlorophenolindophenol, glutathione and catalase Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.27 2,2'-bipyridyl 0.32 mM, 50% inhibition Pseudomonas sp.
1.13.11.27 4-hydroxyphenylpyruvate above 0.2 mM Pseudomonas sp.
1.13.11.27 8-Hydroxyquinoline-5-sulfonic acid 560 nM, 50% inhibition Pseudomonas sp.
1.13.11.27 Bathocuproine 3 mM, 50% inhibition Pseudomonas sp.
1.13.11.27 bathophenanthroline 0.012 mM, 50% inhibition Pseudomonas sp.
1.13.11.27 citrate
-
 Pseudomonas sp.
1.13.11.27 Cupferron 0.0071 mM, 50% inhibition Pseudomonas sp.
1.13.11.27 diethyldithiocarbamate 0.0021 mM, 50% inhibition Pseudomonas sp.
1.13.11.27 EDTA
-
 Pseudomonas sp.
1.13.11.27 H2O2 10 mM, 30% inhibition Pseudomonas sp.
1.13.11.27 Tiron 0.4 mM, 50% inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.27 0.52
-
 phenylpyruvate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.27 copper variable amounts of copper: 0.2-0.5 mol per mol of enzyme Pseudomonas sp.
1.13.11.27 Fe2+ essential role for enzyme bound zinc Pseudomonas sp.
1.13.11.27 Iron variable amounts of iron: 0.6-1.3 mol per mol of enzyme Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.27 36000
-
 4 * 36000, SDS-PAGE Pseudomonas sp.
1.13.11.27 150000
-
 high-speed equilibrium sedimentation Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Pseudomonas sp.
-
-
-
1.13.11.27 Pseudomonas sp. P.J. 874
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.27
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.27 0.92
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.13.11.27 -60°C, 0.2 M NaCl solution buffered with 10 mM potassium phosphate, pH 6.7, stable for several months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Pseudomonas sp. homogentisate + CO2
-
 ?
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Pseudomonas sp. P.J. 874 homogentisate + CO2
-
 ?
1.13.11.27 phenylpyruvate + O2
-
 Pseudomonas sp. 2-hydroxyphenylacetate + CO2
-
 ?
1.13.11.27 phenylpyruvate + O2
-
 Pseudomonas sp. P.J. 874 2-hydroxyphenylacetate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.27 tetramer 4 * 36000, SDS-PAGE Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.27 6 7.5
-
 Pseudomonas sp.