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Literature summary extracted from

  • Lindstedt, S.; Rundgren, M.
    Blue color, metal content, and substrate binding in 4-hydroxyphenylpyruvate dioxygenase from Pseudomonas sp. strain P. J. 874 (1982), J. Biol. Chem., 257, 11922-11931.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.27 1,2-dihydroxybenzene 0.019 mM, 50% inhibition, uncompetitive Pseudomonas sp.
1.13.11.27 1,4-benzoquinone 0.25 mM, 50% inhibition, competitive Pseudomonas sp.
1.13.11.27 1,4-dihydroxybenzene 4.7 mM, 50% inhibition Pseudomonas sp.
1.13.11.27 2,4-Dihydroxybenzoate 4.2 mM, 50% inhibition, noncompetitive Pseudomonas sp.
1.13.11.27 2,5-Dihydroxybenzoate 2.6 mM, 50% inhibition, noncompetitive Pseudomonas sp.
1.13.11.27 2,5-dihydroxyphenylacetate 50% inhibition above 10 mM, noncompetitive Pseudomonas sp.
1.13.11.27 2,5-dihydroxyphenylacetic acid lactone 50% inhibition above 10 mM, competitive Pseudomonas sp.
1.13.11.27 2-hydroxyphenylacetate 10 mM, 50% inhibition, noncompetitive Pseudomonas sp.
1.13.11.27 3,4-dihydroxybenzoate 0.24 mM, 50% inhibition, noncompetitive Pseudomonas sp.
1.13.11.27 3,4-Dihydroxyphenylacetate 0.75 mM, 50% inhibition, noncompetitive Pseudomonas sp.
1.13.11.27 3,5-diiodo-4-hydroxyphenylpyruvate 0.25 mM, 50% inhibition, competitive Pseudomonas sp.
1.13.11.27 3-(2-hydroxyphenyl)propionate 50% inhibition above 10 mM, competitive Pseudomonas sp.
1.13.11.27 3-methoxy-4-hydroxyphenylacetate 0.25 mM, 50% inhibition, competitive Pseudomonas sp.
1.13.11.27 4-hydroxybenzaldehyde 50% inhibition above 10 mM, noncompetitive Pseudomonas sp.
1.13.11.27 4-hydroxyphenylpyruvate substrate inhibition Pseudomonas sp.
1.13.11.27 Acetopyruvate 0.33 mM, 50% inhibition, competitive Pseudomonas sp.
1.13.11.27 bathophenanthroline
-
 Pseudomonas sp.
1.13.11.27 diethyl dicarbonate 5 mM, 50% inhibition Pseudomonas sp.
1.13.11.27 DL-3,4-dihydroxyphenylalanine 0.32 mM, 50% inhibition, noncompetitive Pseudomonas sp.
1.13.11.27 DL-4-hydroxyphenyllactate 50% inhibition above 10 mM, competitive Pseudomonas sp.
1.13.11.27 DL-Epinephrine 0.17 mM, 50% inhibition, noncompetitive Pseudomonas sp.
1.13.11.27 ethanol 5% v/v, 50% inhibition, complete loss of activity at 20% v/v Pseudomonas sp.
1.13.11.27 H2O2 0.01 mM-1 mM, 5-15% inhibition Pseudomonas sp.
1.13.11.27 L-Phe 10 mM, 50% inhibition, competitive Pseudomonas sp.
1.13.11.27 oxaloacetate 10 mM, 50% inhibition, noncompetitive Pseudomonas sp.
1.13.11.27 phenylglyoxalate 50% inhibition above 10 mM, competitive Pseudomonas sp.
1.13.11.27 phenylpyruvate 0.53 mM, 50% inhibition, competitive Pseudomonas sp.
1.13.11.27 pyridoxal 5'-phosphate 2 mM, 50% inhibition Pseudomonas sp.
1.13.11.27 pyruvate 50% inhibition above 10 mM, competitive Pseudomonas sp.
1.13.11.27 trans-4-hydroxycinnamate 50% inhibition above 10 mM, competitive Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.27 Fe2+ the enol tautomer of 4-hydroxyphenylpyruvate appears to form a 2:1 complex with enzyme-bound Fe2+, the tautomerizable keto group with a double bond in 3-4 position favors productive substrate binding to Fe2+ Pseudomonas sp.
1.13.11.27 Iron 0.95 mol of iron per mol of enzyme Pseudomonas sp.
1.13.11.27 Zinc 0.68 mol of zinc per mol of enzyme Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Pseudomonas sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Pseudomonas sp. homogentisate + CO2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.27 37
-
 pH 5.5-8, 15 min, stable Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.27 7.5
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.27 6 9.5 pH 6.0: about 55% of maximal activity, pH 9.5: about 50% of maximal activity Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.27 5.5 8 37°C, 15 min, stable Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.27 additional information
-
 additional information
-
 Pseudomonas sp.