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Literature summary extracted from

  • Lindstedt, S.; Odelhoeg, B.
    4-Hydroxyphenylpyruvate dioxygenase from Pseudomonas (1987), Methods Enzymol., 142, 143-148.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.27 4-hydroxyphenylpyruvate above 0.2 mM; substrate inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.27 0.03
-
 4-hydroxyphenylpyruvate
-
 Pseudomonas sp.
1.13.11.27 0.5
-
 phenylpyruvate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.27 Fe2+ essential role for enzyme-bound Fe2+ in catalytic process Pseudomonas sp.
1.13.11.27 Fe3+ iron in the Fe3+ form must be reduced for enzyme activity Pseudomonas sp.
1.13.11.27 Iron 0.95 mol of iron per mol of enzyme Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.27 36000
-
 4 * 36000, SDS-PAGE Pseudomonas sp.
1.13.11.27 150000
-
 high-speed equilibrium sedimentation Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Pseudomonas sp.
-
-
-
1.13.11.27 Pseudomonas sp. P.J. 874
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.27 1
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.13.11.27 -60°C, stable for several months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Pseudomonas sp. homogentisate + CO2
-
 ?
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Pseudomonas sp. P.J. 874 homogentisate + CO2
-
 ?
1.13.11.27 phenylpyruvate + O2
-
 Pseudomonas sp. 2-hydroxyphenylacetate + CO2
-
 ?
1.13.11.27 phenylpyruvate + O2
-
 Pseudomonas sp. P.J. 874 2-hydroxyphenylacetate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.27 tetramer 4 * 36000, SDS-PAGE Pseudomonas sp.