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Literature summary extracted from

  • Urich, K.
    D-Glutamtoxydase aus der Antennendruse des Flusskrebses Orconectes limosus: Reinigung und Charakterisierung (1968), Z. Naturforsch. B, 23, 1508-1511.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.4.3.7 very stable Faxonius limosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.7 iodoacetamide 64% inhibition at 1 mM Faxonius limosus
1.4.3.7 L-aspartate slight inhibition at 100 mM Faxonius limosus
1.4.3.7 L-glutamate slight inhibition at 100 mM Faxonius limosus
1.4.3.7 p-chloromercuribenzoate 95% inhibition at 0.1 mM Faxonius limosus
1.4.3.7 succinate slight inhibition at 1 mM Faxonius limosus
1.4.3.7 Veronal strong inhibition Faxonius limosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.7 5.4
-
 D-Aspartate
-
 Faxonius limosus
1.4.3.7 15.2
-
 D-glutamate
-
 Faxonius limosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.7 D-aspartate + H2O + O2 Faxonius limosus 20% higher activity with pure oxygen than in air oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.7 D-glutamate + H2O + O2 Faxonius limosus highly specific, no deamination of other D-amino acids found, 30% higher activity with pure oxygen than in air 2-oxoglutarate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.7 Faxonius limosus
-
 crayfish
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.7 partial Faxonius limosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.7 antennal gland
-
 Faxonius limosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.7 0.23
-
-
 Faxonius limosus

Storage Stability

EC Number Storage Stability Organism
1.4.3.7 -30°C, no loss of activity for several months Faxonius limosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.7 D-aspartate + H2O + O2 20% higher activity with pure oxygen than in air Faxonius limosus oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.7 D-glutamate + H2O + O2 highly specific, no deamination of other D-amino acids found, 30% higher activity with pure oxygen than in air Faxonius limosus 2-oxoglutarate + NH3 + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.7 21
-
 stable for more than 5 h Faxonius limosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.7 9
-
 with D-glutamate Faxonius limosus