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Literature summary extracted from

  • Sakuraba, H.; Yoshioka, I.; Koga, S.; Takahashi, M.; Kitahama, Y.; Satomura, T.; Kawakami, R.; Ohshima, T.
    ADP-dependent glucokinase/phosphofructokinase, a novel bifunctional enzyme from the hyperthermophilic archaeon Methanococcus jannaschii (2002), J. Biol. Chem., 277, 12495-12498.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.146 expression in Escherichia coli Methanocaldococcus jannaschii
2.7.1.147 expression in Escherichia coli Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.146 Ca2+
-
 Methanocaldococcus jannaschii
2.7.1.146 Co2+
-
 Methanocaldococcus jannaschii
2.7.1.146 Mg2+
-
 Methanocaldococcus jannaschii
2.7.1.146 Mn2+ 40% of activity Methanocaldococcus jannaschii
2.7.1.146 Ni2+ 40% of activity Methanocaldococcus jannaschii
2.7.1.146 Pb2+ 40% of activity Methanocaldococcus jannaschii
2.7.1.147 Ca2+
-
 Methanocaldococcus jannaschii
2.7.1.147 Co2+ 77% of activity Methanocaldococcus jannaschii
2.7.1.147 Mg2+ required for activation Methanocaldococcus jannaschii
2.7.1.147 Mn2+ 77% of activity Methanocaldococcus jannaschii
2.7.1.147 Ni2+ 38% of activity Methanocaldococcus jannaschii
2.7.1.147 Pb2+ 67% of activity Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.146 53000
-
 1 * 53000, SDS-PAGE Methanocaldococcus jannaschii
2.7.1.146 55000
-
 high pressure liquid chromatography Methanocaldococcus jannaschii
2.7.1.147 53000
-
 1 * 53000, SDS-PAGE Methanocaldococcus jannaschii
2.7.1.147 55000
-
 high pressure liquid chromatography Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.146 D-fructose 6-phosphate + ADP Methanocaldococcus jannaschii ADP can be replaced by GDP and CDP to a limited extent D-fructose 1,6-bisphosphate + AMP
-
 ?
2.7.1.147 D-glucose + ADP Methanocaldococcus jannaschii ADP can be replaced by GDP and CDP to a limited extent D-glucose 6-phosphate + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.146 Methanocaldococcus jannaschii
-
-
-
2.7.1.147 Methanocaldococcus jannaschii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.146 D-fructose 6-phosphate + ADP ADP can be replaced by GDP and CDP to a limited extent Methanocaldococcus jannaschii D-fructose 1,6-bisphosphate + AMP
-
 ?
2.7.1.147 D-glucose + ADP ADP can be replaced by GDP and CDP to a limited extent Methanocaldococcus jannaschii D-glucose 6-phosphate + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.146 monomer 1 * 53000, SDS-PAGE Methanocaldococcus jannaschii
2.7.1.147 monomer 1 * 53000, SDS-PAGE Methanocaldococcus jannaschii