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Literature summary extracted from
- Nitrate reductase from Achromobacter fischeri. Purification and properties: Function of flavines and cytochrome (1957), Arch. Biochem. Biophys., 67, 16-34.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.9.6.1 | stable to prolonged dialysis | Aliivibrio fischeri |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.9.6.1 | azide | NaN3 at 30 mM completely inactivates | Aliivibrio fischeri |  |
| 1.9.6.1 | hydrogensulfite | 0.1 mM, complete inhibition | Aliivibrio fischeri | |
| 1.9.6.1 | p-chloromercuribenzoate | 0.3 mM, completely inhibits, can be reversed by cysteine or glutathione | Aliivibrio fischeri | |
| 1.9.6.1 | Sulfide | Na2S at 0.5 mM inactivates completely | Aliivibrio fischeri | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.9.6.1 | additional information | - |
additional information | - |
Aliivibrio fischeri |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.9.6.1 | soluble | - |
Aliivibrio fischeri | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.9.6.1 | Iron | contains iron | Aliivibrio fischeri | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.9.6.1 | 100000 | - |
- |
Aliivibrio fischeri |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.9.6.1 | nitrate + reduced acceptor | Aliivibrio fischeri | the enzymes EC 1.7.99.4 and EC 1.9.6.1 are probably identical, in vivo cytochrome serves as electron donor in the electron transport chain to nitrate | nitrite + acceptor | - |
? | |
| 1.9.6.1 | nitrate + reduced acceptor | Aliivibrio fischeri | electron transport chain in vivo: Fe3 + via specific cytochrome-nitrate reductase to NO3- | nitrite + acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.9.6.1 | Aliivibrio fischeri | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.9.6.1 | - |
Aliivibrio fischeri |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.9.6.1 | 1.484 | - |
- |
Aliivibrio fischeri |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.9.6.1 | frozen, several months, stable | Aliivibrio fischeri |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.9.6.1 | additional information | the enzymes EC 1.7.99.4 and EC 1.9.6.1 are probably identical, in vivo cytochrome serves as electron donor in the electron transport chain to nitrate | Aliivibrio fischeri | ? | - |
? | |
| 1.9.6.1 | nitrate + reduced acceptor | reduced benzyl viologen as electron donor | Aliivibrio fischeri | nitrite + acceptor | - |
? | |
| 1.9.6.1 | nitrate + reduced acceptor | reduced methyl viologen as electron donor | Aliivibrio fischeri | nitrite + acceptor | - |
? | |
| 1.9.6.1 | nitrate + reduced acceptor | the enzymes EC 1.7.99.4 and EC 1.9.6.1 are probably identical, in vivo cytochrome serves as electron donor in the electron transport chain to nitrate | Aliivibrio fischeri | nitrite + acceptor | - |
? | |
| 1.9.6.1 | nitrate + reduced acceptor | electron transport chain in vivo: Fe3 + via specific cytochrome-nitrate reductase to NO3- | Aliivibrio fischeri | nitrite + acceptor | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.9.6.1 | More | the enzymes EC 1.7.99.4 and EC 1.9.6.1 are probably identical, in vivo cytochrome serves as electron donor in the electron transport chain to nitrate | Aliivibrio fischeri |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.9.6.1 | 50 | - |
10 min, stable | Aliivibrio fischeri |
| 1.9.6.1 | 60 | - |
10 min, 10-15% loss of activity | Aliivibrio fischeri |
| 1.9.6.1 | 70 | - |
5 min, complete and irreversible loss of activity | Aliivibrio fischeri |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.9.6.1 | 7 | 8.5 | same rate between | Aliivibrio fischeri |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.9.6.1 | 5.5 | 10 | sharp drop of activity below pH 5.5 and above pH 10 | Aliivibrio fischeri |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.9.6.1 | cytochrome | presence of a bound cytochrome | Aliivibrio fischeri | |
| 1.9.6.1 | additional information | enzyme contains no flavin | Aliivibrio fischeri |
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