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Literature summary extracted from

  • Suylen, G.M.H.; Large, P.J.; van Dijken, J.P.; Kuenen, J.G.
    Methyl mercaptan oxidase, a key enzyme in the metabolism of methylated sulphur compounds by Hyphomicrobium EG (1987), J. Gen. Microbiol., 133, 2989-2997.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.3.4 (NH4)2SO4
-
 Hyphomicrobium sp.
1.8.3.4 cyanide
-
 Hyphomicrobium sp.
1.8.3.4 ethyl mercaptan
-
 Hyphomicrobium sp.
1.8.3.4 methanethiol above 0.014 mM Hyphomicrobium sp.
1.8.3.4 methylamine
-
 Hyphomicrobium sp.
1.8.3.4 NH4+
-
 Hyphomicrobium sp.
1.8.3.4 NH4Cl
-
 Hyphomicrobium sp.
1.8.3.4 Sulfide non-competitive inhibition of methyl mercaptan oxidation Hyphomicrobium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.4 0.005 0.01 methanethiol
-
 Hyphomicrobium sp.
1.8.3.4 0.018
-
 ethyl mercaptan
-
 Hyphomicrobium sp.
1.8.3.4 0.06
-
 Sulfide
-
 Hyphomicrobium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.4 40000 50000 gel filtration Hyphomicrobium sp.
1.8.3.4 49000
-
 1 * 49000, SDS-PAGE Hyphomicrobium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.4 Hyphomicrobium sp.
-
-
-
1.8.3.4 Hyphomicrobium sp. EG
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.4
-
 Hyphomicrobium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.3.4 2.13
-
-
 Hyphomicrobium sp.

Storage Stability

EC Number Storage Stability Organism
1.8.3.4 -20°C, stable for months Hyphomicrobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.4 ethyl mercaptan + O2 + H2O
-
 Hyphomicrobium sp. acetaldehyde + H2S + H2O2
-
 ?
1.8.3.4 ethyl mercaptan + O2 + H2O
-
 Hyphomicrobium sp. EG acetaldehyde + H2S + H2O2
-
 ?
1.8.3.4 methanethiol + O2 + H2O
-
 Hyphomicrobium sp. formaldehyde + H2S + H2O2
-
 ?
1.8.3.4 methanethiol + O2 + H2O
-
 Hyphomicrobium sp. EG formaldehyde + H2S + H2O2
-
 ?
1.8.3.4 sulfide + O2
-
 Hyphomicrobium sp. sulfur + H2O2
-
 ?
1.8.3.4 sulfide + O2
-
 Hyphomicrobium sp. EG sulfur + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.3.4 monomer 1 * 49000, SDS-PAGE Hyphomicrobium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.3.4 50
-
 10 min, stable Hyphomicrobium sp.
1.8.3.4 65
-
 10 min, complete loss of activity Hyphomicrobium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.4 8.2
-
-
 Hyphomicrobium sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.3.4 0.04
-
 cyanide
-
 Hyphomicrobium sp.
1.8.3.4 0.042
-
 methanethiol
-
 Hyphomicrobium sp.
1.8.3.4 0.09
-
 Sulfide
-
 Hyphomicrobium sp.
1.8.3.4 1.4
-
 methylamine
-
 Hyphomicrobium sp.
1.8.3.4 4
-
 NH4+
-
 Hyphomicrobium sp.