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Literature summary extracted from
- Conversion of the central [4Fe-4S] cluster into a [3Fe-4S] cluster leads to reduced hydrogen-uptake activity of the F420-reducing hydrogenase of Methanococcus voltae (2000), Eur. J. Biochem., 267, 6612-6618.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.12.98.1 | - |
Methanococcus voltae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.12.98.1 | C205P | no effect on benzyl viologen reduction, 10 fold decrease in F420 reduction, wild type enzyme contains 3[4Fe-4S], mutant enzyme contains 1[3Fe-4S] | Methanococcus voltae |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.98.1 | Iron | iron-sulfur cluster | Methanococcus voltae |  |
| 1.12.98.1 | sulfur | iron-sulfur cluster | Methanococcus voltae | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.98.1 | H2 + coenzyme F420 | Methanococcus voltae | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.98.1 | Methanococcus voltae | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.1 | 28 | - |
C205P mutant enzyme with F420 | Methanococcus voltae |
| 1.12.98.1 | 345 | - |
native enzyme with benzyl viologen | Methanococcus voltae |
| 1.12.98.1 | 364 | - |
native enzyme with F420 | Methanococcus voltae |
| 1.12.98.1 | 540 | - |
C205P mutant enzyme with benzyl viologen | Methanococcus voltae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.98.1 | H2 + benzyl viologen | - |
Methanococcus voltae | ? | - |
? | |
| 1.12.98.1 | H2 + coenzyme F420 | - |
Methanococcus voltae | reduced coenzyme F420 | - |
? | |
| 1.12.98.1 | H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanococcus voltae | reduced coenzyme F420 | - |
? | |
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Release 2023.1 (January 2023)
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