EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.12.98.1 | additional information | 2 times higher activity in biological buffers like Tris or EPPS compared with phosphate buffer | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 115000 | - |
smallest species with enzymatic activity, native SDS-PAGE | Methanocaldococcus jannaschii |
1.12.98.1 | 990000 | - |
native SDS-PAGE | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.98.1 | H2 + coenzyme F420 | Methanocaldococcus jannaschii | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.98.1 | Methanocaldococcus jannaschii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.98.1 | partial | Methanocaldococcus jannaschii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 5.2 | - |
purified enzyme with F420 | Methanocaldococcus jannaschii |
1.12.98.1 | 16 | - |
purified enzyme with methyl viologen | Methanocaldococcus jannaschii |
EC Number | Storage Stability | Organism |
---|---|---|
1.12.98.1 | -70°C, loss of high temperature activity | Methanocaldococcus jannaschii |
1.12.98.1 | 4°C, 5% loss of activity within 24 h | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.98.1 | H2 + coenzyme F420 | - |
Methanocaldococcus jannaschii | reduced coenzyme F420 | - |
? | |
1.12.98.1 | H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanocaldococcus jannaschii | reduced coenzyme F420 | - |
? | |
1.12.98.1 | H2 + methyl viologen | - |
Methanocaldococcus jannaschii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.98.1 | multimer | alpha,beta,gamma 48000 + 32000 + 25000, SDS-PAGE, exact subunit composition not known | Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 80 | 90 | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 25 | 90 | F420 reduction | Methanocaldococcus jannaschii |
1.12.98.1 | 40 | 103 | at 103°C low activity with methyl viologen | Methanocaldococcus jannaschii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 70 | - |
stable for more than 3 h, methyl viologen-linked activity more stable against heating than F420-linked activity | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 7 | - |
F420 reduction | Methanocaldococcus jannaschii |
1.12.98.1 | 10 | - |
optimum pH for methyl viologen reduction above pH 10 | Methanocaldococcus jannaschii |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 10 | - |
unstable above pH 10 | Methanocaldococcus jannaschii |