Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Alex, L.A.; Reeve, J.N.; Orme-Johnson, W.H.; Walsh, C.T.
    Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H (1990), Biochemistry, 29, 7237-7244.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.98.1 expressed in Escherichia coli Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.1 Iron iron-sulfur cluster Methanothermobacter thermautotrophicus
1.12.98.1 Nickel
-
Methanothermobacter thermautotrophicus
1.12.98.1 sulfur iron-sulfur cluster Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + coenzyme F420 Methanothermobacter thermautotrophicus enzyme of methanogenesis pathway reduced coenzyme F420
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanothermobacter thermautotrophicus P19496 and P19499 and P19498 strain delta H
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.1
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + coenzyme F420
-
Methanothermobacter thermautotrophicus reduced coenzyme F420
-
?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanothermobacter thermautotrophicus reduced coenzyme F420
-
?

Subunits

EC Number Subunits Comment Organism
1.12.98.1 multimer alpha,beta,gamma,delta 44700 + 30700 + 25700 + 17600, calculated from DNA sequence, SDS-PAGE, delta subunit not visible in SDS-PAGE, exact subunit composition not known, function of delta subunit not known Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.98.1 FAD
-
Methanothermobacter thermautotrophicus