Any feedback?
Literature summary extracted from
- Purification and properties of the membrane-associated coenzyme F420-reducing hydrogenase from Methanobacterium formicicum (1989), J. Bacteriol., 171, 3846-3853.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.98.1 | FAD | reactivation without added FAD restores only methyl viologen-dependent activity, no activity with F420 | Methanobacterium formicicum |  |
| 1.12.98.1 | KCl | 0.8 M activate F420-linked activity more than 10fold, slight inhibition of methyl viologen linked activity | Methanobacterium formicicum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.12.98.1 | 0.037 | - |
coenzyme F420 | - |
Methanobacterium formicicum | |
| 1.12.98.1 | 0.08 | - |
coenzyme F0 | - |
Methanobacterium formicicum | |
| 1.12.98.1 | 0.087 | - |
FAD | - |
Methanobacterium formicicum | |
| 1.12.98.1 | 1.56 | - |
methyl viologen | - |
Methanobacterium formicicum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.98.1 | Iron | - |
Methanobacterium formicicum | |
| 1.12.98.1 | Nickel | - |
Methanobacterium formicicum | |
| 1.12.98.1 | selenium | no selenium detected | Methanobacterium formicicum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.1 | 102000 | - |
smallest active species, gel filtration | Methanobacterium formicicum |
| 1.12.98.1 | 109000 | - |
gel filtration | Methanobacterium formicicum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.98.1 | H2 + coenzyme F420 | Methanobacterium formicicum | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.98.1 | Methanobacterium formicicum | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.12.98.1 | purified enzyme requires reductive reactivation before assay, reactivation possible with H2 or glucose and glucose oxidase | Methanobacterium formicicum |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.12.98.1 | - |
Methanobacterium formicicum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.1 | 97.6 | - |
purified enzyme with F420 | Methanobacterium formicicum |
| 1.12.98.1 | 98.6 | - |
purified enzyme with F0 | Methanobacterium formicicum |
| 1.12.98.1 | 172 | - |
purified enzyme with methyl viologen | Methanobacterium formicicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.98.1 | H2 + coenzyme F0 | - |
Methanobacterium formicicum | reduced coenzyme F0 | - |
? | |
| 1.12.98.1 | H2 + coenzyme F420 | - |
Methanobacterium formicicum | reduced coenzyme F420 | - |
r | |
| 1.12.98.1 | H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanobacterium formicicum | reduced coenzyme F420 | - |
r | |
| 1.12.98.1 | H2 + FAD | - |
Methanobacterium formicicum | H+ + FADH2 | - |
r | |
| 1.12.98.1 | H2 + methyl viologen | - |
Methanobacterium formicicum | ? | - |
? | |
| 1.12.98.1 | additional information | reduces different flavins | Methanobacterium formicicum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.12.98.1 | multimer | alpha,beta,gamma 43600 + 36700 + 28000, SDS-PAGE, exact subunit composition not known, ratio 1:1:1 postulated | Methanobacterium formicicum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.1 | 55 | - |
- |
Methanobacterium formicicum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.1 | 7 | 7.5 | - |
Methanobacterium formicicum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.1 | 5.5 | 9.5 | - |
Methanobacterium formicicum |
| 1.12.98.1 | 5.5 | 8 | reverse reaction | Methanobacterium formicicum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.98.1 | FAD | 1 mol per mol 109000 Da species | Methanobacterium formicicum | |
| 1.12.98.1 | FAD | FAD depleted enzyme reduces methyl viologen but not F420 | Methanobacterium formicicum | |
| 1.12.98.1 | additional information | no activity with FMN after FAD depletion | Methanobacterium formicicum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
