Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Livingston, D.J.; Fox, J.A.; Orme-Johnson, W.H.; Walsh, C.T.
    8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 2. Kinetic and hydrogen-transfer studies (1987), Biochemistry, 26, 4228-4237.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.98.1 Procion Blue HB stimulates methyl viologen reduction Methanothermobacter thermautotrophicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.98.1 CO dead end inhibitor, competitive for H2, uncompetetitive versus F0 Methanothermobacter thermautotrophicus
1.12.98.1 coenzyme F0 substrate inhibition above 0.05 mM Methanothermobacter thermautotrophicus
1.12.98.1 Procion Blue HB inhibits F0 reduction Methanothermobacter thermautotrophicus
1.12.98.1 reduced coenzyme F0 noncompetitive product inhibition Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.98.1 0.012
-
 H2
-
 Methanothermobacter thermautotrophicus
1.12.98.1 0.026
-
 coenzyme F0
-
 Methanothermobacter thermautotrophicus
1.12.98.1 0.036
-
 coenzyme F420
-
 Methanothermobacter thermautotrophicus
1.12.98.1 0.1
-
 reduced coenzyme F0 anaerobic conditions Methanothermobacter thermautotrophicus
1.12.98.1 1.5
-
 methyl viologen
-
 Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.1 Iron iron-sulfur cluster Methanothermobacter thermautotrophicus
1.12.98.1 Iron iron-sulfur cluster Methanococcus voltae
1.12.98.1 Nickel
-
 Methanothermobacter thermautotrophicus
1.12.98.1 Nickel
-
 Methanococcus voltae
1.12.98.1 sulfur iron-sulfur cluster Methanothermobacter thermautotrophicus
1.12.98.1 sulfur iron-sulfur cluster Methanococcus voltae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + coenzyme F420 Methanothermobacter thermautotrophicus enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 Methanococcus voltae enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanococcus voltae
-
-
-
1.12.98.1 Methanothermobacter thermautotrophicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + coenzyme F0
-
 Methanothermobacter thermautotrophicus reduced coenzyme F0
-
 r
1.12.98.1 H2 + coenzyme F420
-
 Methanothermobacter thermautotrophicus reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420
-
 Methanococcus voltae reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanothermobacter thermautotrophicus reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanococcus voltae reduced coenzyme F420
-
 ?
1.12.98.1 H2 + methyl viologen
-
 Methanothermobacter thermautotrophicus ?
-
 ?
1.12.98.1 H2 + methyl viologen
-
 Methanococcus voltae ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12.98.1 2050
-
 coenzyme F420
-
 Methanothermobacter thermautotrophicus
1.12.98.1 2250
-
 methyl viologen
-
 Methanothermobacter thermautotrophicus
1.12.98.1 2250
-
 coenzyme F0 equal turnover in reverse reaction under anaerobic conditions Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.98.1 FAD
-
 Methanothermobacter thermautotrophicus
1.12.98.1 FAD
-
 Methanococcus voltae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.12.98.1 0.087
-
 coenzyme F0
-
 Methanothermobacter thermautotrophicus