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Literature summary extracted from

  • Fox, J.A.; Livingston, D.J.; Orme-Johnson, W.H.; Walsh, C.T.
    8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization (1987), Biochemistry, 26, 4219-4227.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.1 Iron iron-sulfur cluster Methanothermobacter thermautotrophicus
1.12.98.1 sulfur iron-sulfur cluster Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.1 26000
-
 alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE Methanothermobacter thermautotrophicus
1.12.98.1 31000
-
 alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE Methanothermobacter thermautotrophicus
1.12.98.1 47000
-
 alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE Methanothermobacter thermautotrophicus
1.12.98.1 115000
-
 smallest species with enzymatic activity, native SDS-PAGE Methanothermobacter thermautotrophicus
1.12.98.1 800000
-
 gel filtration, native SDS-PAGE Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + coenzyme F420 Methanothermobacter thermautotrophicus enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanothermobacter thermautotrophicus
-
 strain delta H
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.98.1 aerobic purification yields the stable but inactive enzyme, can be reactivated under reducing conditions Methanothermobacter thermautotrophicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.1 aerobic purification causes reversible inhibition with O2 Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.1 49
-
 purified enzyme, F0-linked activity Methanothermobacter thermautotrophicus

Storage Stability

EC Number Storage Stability Organism
1.12.98.1 -20°C, 1 year, inactive form maintains 70% of initial activity after reactivation Methanothermobacter thermautotrophicus
1.12.98.1 room temperature, activated enzyme loses 20% of inital activity within 24 h Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + coenzyme F0
-
 Methanothermobacter thermautotrophicus reduced coenzyme F0
-
 ?
1.12.98.1 H2 + coenzyme F420
-
 Methanothermobacter thermautotrophicus reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanothermobacter thermautotrophicus reduced coenzyme F420
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.1 multimer alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE Methanothermobacter thermautotrophicus
1.12.98.1 multimer ratio alpha, beta, gamma postulated to be 1:1:1 Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.98.1 6.5 7.5 F420 reduction Methanothermobacter thermautotrophicus
1.12.98.1 11
-
 optimum pH for methyl viologen reduction above pH 11 Methanothermobacter thermautotrophicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.12.98.1 5 9 F420 reduction Methanothermobacter thermautotrophicus
1.12.98.1 7 11 increasing methyl viologen activity Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.98.1 FAD 0.8-0.9 mol per mol 115000 Da species, bound to gamma subunit Methanothermobacter thermautotrophicus