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Literature summary extracted from
- Locations of the hydrogenases of Methanobacterium formicicum after subcellular fractionation of cell extract (1987), J. Bacteriol., 169, 3823-3825.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.98.1 | FAD | restores activity after depletion of FAD in hydrophobic interaction chromatography | Methanobacterium formicicum |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.12.98.1 | 0.03 mM FAD stabilizes enzyme | Methanobacterium formicicum |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.12.98.1 | membrane | associated | Methanobacterium formicicum | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.1 | 790000 | - |
biggest enzyme species, aggregate | Methanobacterium formicicum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.98.1 | H2 + coenzyme F420 | Methanobacterium formicicum | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.98.1 | Methanobacterium formicicum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.12.98.1 | - |
Methanobacterium formicicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.98.1 | H2 + coenzyme F420 | - |
Methanobacterium formicicum | reduced coenzyme F420 | - |
? | |
| 1.12.98.1 | H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanobacterium formicicum | reduced coenzyme F420 | - |
? | |
| 1.12.98.1 | H2 + methyl viologen | - |
Methanobacterium formicicum | ? | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.98.1 | FAD | bound to protein, depleted by hydrophobic interaction chromatography | Methanobacterium formicicum | |
| 1.12.98.1 | FAD | FAD depleted enzyme reduces methyl viologen but not F420 | Methanobacterium formicicum | |
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