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Literature summary extracted from

  • Cutruzzola, f.; Arese, M.; Grasso, S.; Bellelli, A.; Brunori, M.
    Mutagenesis of nitrite reductase from Pseudomonas aeruginosa: tyrosine-10 in the c heme domain is not involved in catalysis (1997), FEBS Lett., 412, 365-369.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.1 expression of wild-type and Y10F mutant enzyme in Pseudomonas putida Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.7.2.1 Y10F no change in optical spectrum, nitrite and oxidase activity and heme c to heme d1 electron transfer rates compared to wild-type Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.1 0.00084
-
 azurin recombinant Y10F mutant enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa
1.7.2.1 0.00177
-
 ferricytochrome c551 recombinant wild-type enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa
1.7.2.1 0.035
-
 azurin recombinant wild-type and Y10F mutant enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.1 recombinant wild-type and Y10F mutant enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 nitric oxide + H2O + ferricytochrome c551
-
 Pseudomonas aeruginosa nitrite + ferrocytochrome c551 + H+
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.1 0.583
-
 ferricytochrome c551 recombinant wild-type enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa
1.7.2.1 0.6
-
 ferricytochrome c551 recombinant Y10F mutant enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa
1.7.2.1 1
-
 azurin recombinant Y10F mutant enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa
1.7.2.1 1.12
-
 azurin recombinant wild-type enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa