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Literature summary extracted from

  • Little, H.N.
    Oxidation of nitroethane by extracts from Neurospora (1951), J. Biol. Chem., 193, 347-358.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.3.1 dialysis: stable to Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.1 Boric acid
-
 Neurospora crassa
1.7.3.1 CuSO4
-
 Neurospora crassa
1.7.3.1 KNO3
-
 Neurospora crassa
1.7.3.1 Maleic acid
-
 Neurospora crassa
1.7.3.1 NaCl slight Neurospora crassa
1.7.3.1 NaCN slight Neurospora crassa
1.7.3.1 NH2OH slight Neurospora crassa
1.7.3.1 Tris
-
 Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.3.1 nitroethane + H2O + O2 Neurospora crassa
-
 acetaldehyde + nitrite + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.1 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.1 partial Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.3.1 mycelium
-
 Neurospora crassa
-

Storage Stability

EC Number Storage Stability Organism
1.7.3.1 -20°C, weeks, slow decrease of activity Neurospora crassa
1.7.3.1 in refrigerator for several days Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.1 1-nitropropane + H2O + O2
-
 Neurospora crassa propionaldehyde + nitrite + H2O2
-
 ?
1.7.3.1 2-nitropropane + H2O + O2
-
 Neurospora crassa acetone + nitrite + H2O2
-
 ?
1.7.3.1 3-nitropropanoic acid + H2O + O2
-
 Neurospora crassa 2-oxopropanoic acid + nitrite + H2O2
-
 ?
1.7.3.1 additional information slight activity, less than 5% of the activity towards nitroethane with: nitromethane, nitroacetic acid, 1-chloro-1-nitropropane, methylene blue also can function as hydrogen acceptor with low reaction rate Neurospora crassa ?
-
 ?
1.7.3.1 nitroethane + H2O + O2
-
 Neurospora crassa acetaldehyde + nitrite + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.3.1 50
-
 10 s, 70% loss of activity Neurospora crassa
1.7.3.1 100
-
 2 min, complete loss of activity Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.1 7
-
-
 Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.3.1 6.2 7.6 half-maximal activity at pH 6.2 and 7.6 Neurospora crassa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.3.1 5.5
-
 1 h, 0°C, complete loss of activity Neurospora crassa
1.7.3.1 11
-
 1 h, 0°C, full activity recovered after readjusting the solution to pH 7.0 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.3.1 additional information probably not a hemoprotein Neurospora crassa