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Literature summary extracted from

  • Wang, C.L.; Takenaka, S.; Murakami, S.; Aoki, K.
    Production of catechol from benzoate by the wild strain Ralstonia species Ba-0323 and characterization of its catechol 1,2-dioxygenase (2001), Biosci. Biotechnol. Biochem., 65, 1957-1964.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 Ag+
-
 Ralstonia sp.
1.13.11.1 Cu2+
-
 Ralstonia sp.
1.13.11.1 p-chloromercuribenzoate
-
 Ralstonia sp.
1.13.11.1 Tiron
-
 Ralstonia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.0037
-
 3-methylcatechol
-
 Ralstonia sp.
1.13.11.1 0.013
-
 catechol
-
 Ralstonia sp.
1.13.11.1 0.014
-
 4-methylcatechol
-
 Ralstonia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 30000
-
 1 * 30000, SDS-PAGE Ralstonia sp.
1.13.11.1 31000
-
 gel filtration Ralstonia sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Ralstonia sp.
-
 Ba-0323
-
1.13.11.1 Ralstonia sp. Ba-0323
-
 Ba-0323
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
 Ralstonia sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 additional information
-
-
 Ralstonia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2
-
 Ralstonia sp. (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 3-methylcatechol + O2
-
 Ralstonia sp. Ba-0323 (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Ralstonia sp. (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Ralstonia sp. Ba-0323 (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 catechol + O2
-
 Ralstonia sp. cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Ralstonia sp. Ba-0323 cis,cis-muconate
-
 ?
1.13.11.1 additional information no intradiol cleavage of 3- and 4-chlorocatechol or 4-fluorocatechol, no substrates: protocatechuic acid Ralstonia sp. ?
-
 ?
1.13.11.1 additional information no intradiol cleavage of 3- and 4-chlorocatechol or 4-fluorocatechol, no substrates: protocatechuic acid Ralstonia sp. Ba-0323 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 monomer 1 * 30000, SDS-PAGE Ralstonia sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.1 0.4
-
 3-methylcatechol
-
 Ralstonia sp.
1.13.11.1 1.6
-
 4-methylcatechol
-
 Ralstonia sp.
1.13.11.1 9.6
-
 catechol
-
 Ralstonia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 8
-
-
 Ralstonia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.1 7.5 9
-
 Ralstonia sp.