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Literature summary extracted from

  • Sauret-Ignazi, G.; Gagnon, J.; Beguin, C.; Barrelle, M.; Markowicz, J.; Pelmont, J.; Toussaint, A.
    Characterization of a chromosomally encoded catechol 1,2-dioxygenase (E.C. 1.13.11.1) from Alcaligenes eutrophus CH34 (1996), Arch. Microbiol., 166, 42-50.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 3,4,5-trichlorocatechol competitive inhibitor Cupriavidus necator
1.13.11.1 3-methylcatechol at concentration at 20 microM and above Cupriavidus necator
1.13.11.1 4,5-dichlorocatechol competitive inhibitor Cupriavidus necator
1.13.11.1 tetrachlorocatechol markedly inhibitory at concentration above 4 microM Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.00025
-
 catechol
-
 Cupriavidus necator
1.13.11.1 0.0005
-
 4-Chlorocatechol
-
 Cupriavidus necator
1.13.11.1 0.0005
-
 tetrachlorocatechol
-
 Cupriavidus necator
1.13.11.1 0.0007
-
 4-Fluorocatechol
-
 Cupriavidus necator
1.13.11.1 0.002
-
 3-chlorocatechol
-
 Cupriavidus necator
1.13.11.1 0.009
-
 3-methylcatechol
-
 Cupriavidus necator
1.13.11.1 0.018
-
 4-methylcatechol
-
 Cupriavidus necator
1.13.11.1 0.1
-
 oxygen
-
 Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Iron 0.83 mol per mol of protein Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 36000
-
 2 * 36000, SDS-PAGE Cupriavidus necator
1.13.11.1 76000
-
 gel filtration Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Cupriavidus necator
-
 Ralstonia eutropha CH34
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
 Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 9.36
-
-
 Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-chlorocatechol + O2
-
 Cupriavidus necator 2-chloro-cis,cis-muconate
-
 ?
1.13.11.1 3-methylcatechol + O2
-
 Cupriavidus necator (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-chlorocatechol + O2
-
 Cupriavidus necator (2E,4Z)-3-chlorohexa-2,4-dienedioate
-
 ?
1.13.11.1 4-fluorocatechol + O2
-
 Cupriavidus necator 3-fluoro-cis,cis-muconate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Cupriavidus necator (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 catechol + O2
-
 Cupriavidus necator cis,cis-muconate
-
 ?
1.13.11.1 tetrachlorocatechol + O2
-
 Cupriavidus necator cis,cis-tetrachloromuconic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 dimer 2 * 36000, SDS-PAGE Cupriavidus necator

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.1 1
-
 4-Chlorocatechol
-
 Cupriavidus necator
1.13.11.1 4
-
 4-methylcatechol
-
 Cupriavidus necator
1.13.11.1 4
-
 4-Fluorocatechol
-
 Cupriavidus necator
1.13.11.1 20
-
 catechol
-
 Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 8.5
-
-
 Cupriavidus necator