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Literature summary extracted from

  • Aoki, K.; Konohana, T.; Shinke, R.; Nishira, H.
    Purification and characterization of catechol 1,2-dioxygenase from aniline-assimilating Rhodococcus erythropolis AN-13 (1984), Agric. Biol. Chem., 48, 2087-2095.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 Ag+
-
Rhodococcus erythropolis
1.13.11.1 Fe2+
-
Rhodococcus erythropolis
1.13.11.1 Fe3+
-
Rhodococcus erythropolis
1.13.11.1 Hg2+
-
Rhodococcus erythropolis
1.13.11.1 iodoacetic acid
-
Rhodococcus erythropolis
1.13.11.1 o-phenanthroline
-
Rhodococcus erythropolis
1.13.11.1 p-chloromercuribenzoate
-
Rhodococcus erythropolis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Iron 1.3 gatom per mol enzyme protein Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 36000
-
4 * 36000, SDS-PAGE Rhodococcus erythropolis
1.13.11.1 37000
-
both CD I and CD II, buffer containing NaCl, gel filtration Rhodococcus erythropolis
1.13.11.1 150000
-
buffer omitting NaCl, gel filtration Rhodococcus erythropolis

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Rhodococcus erythropolis
-
AN-13
-
1.13.11.1 Rhodococcus erythropolis AN-13
-
AN-13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
Rhodococcus erythropolis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 24
-
catechol Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2
-
Rhodococcus erythropolis (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
?
1.13.11.1 3-methylcatechol + O2
-
Rhodococcus erythropolis AN-13 (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
?
1.13.11.1 4-methylcatechol + O2
-
Rhodococcus erythropolis (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
?
1.13.11.1 4-methylcatechol + O2
-
Rhodococcus erythropolis AN-13 (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
?
1.13.11.1 catechol + O2
-
Rhodococcus erythropolis cis,cis-muconate
-
?
1.13.11.1 catechol + O2
-
Rhodococcus erythropolis AN-13 cis,cis-muconate
-
?
1.13.11.1 additional information intradiol cleavage of 3- and 4-methylcatechol, but not extradiol cleavage of 3-methylcatechol Rhodococcus erythropolis ?
-
?
1.13.11.1 additional information intradiol cleavage of 3- and 4-methylcatechol, but not extradiol cleavage of 3-methylcatechol Rhodococcus erythropolis AN-13 ?
-
?
1.13.11.1 pyrogallol + O2
-
Rhodococcus erythropolis alpha-hydroxymuconic acid
-
?
1.13.11.1 pyrogallol + O2
-
Rhodococcus erythropolis AN-13 alpha-hydroxymuconic acid
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 tetramer 4 * 36000, SDS-PAGE Rhodococcus erythropolis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.1 40
-
for 10 min Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7.5
-
for catechol as substrate Rhodococcus erythropolis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.1 7 10.5
-
Rhodococcus erythropolis