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Literature summary extracted from

  • Hayaishi, O.
    Direct oxygenation by O2. Oxygenases (1963), The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. ), 8, 353-371.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 Ag+ inhibition can almost completely be reversed by addition of glutathione Pseudomonas fluorescens
1.13.11.1 Cu2+
-
Pseudomonas fluorescens
1.13.11.1 Hg2+ inhibition can almost completely reserved by addition of glutathione Pseudomonas fluorescens
1.13.11.1 p-chloromercuribenzoate inhibition can almost completely be reversed by addition of glutathione Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.0005
-
catechol
-
Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Iron 2 atoms per molecule of enzyme protein Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 83000
-
-
Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Pseudomonas fluorescens
-
ATCC 11250
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 additional information
-
-
Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
1.13.11.1 -10°C, pH 9.0, 5-10% loss of activity after several months Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 4-methylcatechol + O2
-
Pseudomonas fluorescens (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
?
1.13.11.1 catechol + O2
-
Pseudomonas fluorescens cis,cis-muconate
-
?
1.13.11.1 additional information no substrates: adrenolutine, p-aminocatechol, protocatechuic acid, 2,3-dihydroxybenzoic acid, 2,4-dihydroxybenzoic acid, trans-5,6-dihydroxycyclohexadiene, pyrogallol, dopa, homogentisic acid, gentisic acid Pseudomonas fluorescens ?
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.1 26.7
-
catechol
-
Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7 8 phosphate buffer Pseudomonas fluorescens
1.13.11.1 7 10 phosphate buffer, addition of glutathione Pseudomonas fluorescens