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Literature summary extracted from

  • Mori, N.; Kawakami, B.; Tani, Y.; Yamada, H.
    Purification and properties of dimethylglycine oxidase from Cylindrocarpon didymum M-1 (1980), Agric. Biol. Chem., 44, 1383-1389.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.10 5,5-dithio-bis(2-nitrobenzoic acid) 28% inhibition at 1 mM Cylindrocarpon didymum
1.5.3.10 Ag+ 45% inhibition at 1 mM Cylindrocarpon didymum
1.5.3.10 Hg2+ 89% inhibition at 1 mM Cylindrocarpon didymum
1.5.3.10 iodoacetate complete inhibition at 1 mM Cylindrocarpon didymum
1.5.3.10 Mn2+ 30% inhibition at 1 mM Cylindrocarpon didymum
1.5.3.10 Zn2+ 51% inhibition at 1 mM Cylindrocarpon didymum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.10 9.1
-
 N,N-dimethylglycine
-
 Cylindrocarpon didymum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.10 82000
-
 2 * 82000, SDS-PAGE Cylindrocarpon didymum
1.5.3.10 170000
-
 gel filtration Cylindrocarpon didymum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.10 N,N-dimethylglycine + H2O + O2 Cylindrocarpon didymum
-
 sarcosine + formaldehyde + H2O2
-
 ?
1.5.3.10 N,N-dimethylglycine + H2O + O2 Cylindrocarpon didymum M-1
-
 sarcosine + formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.10 Cylindrocarpon didymum
-
-
-
1.5.3.10 Cylindrocarpon didymum M-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.10 to homogeneity, chromatography steps Cylindrocarpon didymum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.10 cell culture
-
 Cylindrocarpon didymum
-
1.5.3.10 hepatocyte
-
 Cylindrocarpon didymum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.10 12.3
-
-
 Cylindrocarpon didymum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.10 N,N-dimethylglycine + H2O + O2
-
 Cylindrocarpon didymum sarcosine + formaldehyde + H2O2
-
 ?
1.5.3.10 N,N-dimethylglycine + H2O + O2
-
 Cylindrocarpon didymum M-1 sarcosine + formaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.10 dimer 2 * 82000, SDS-PAGE Cylindrocarpon didymum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.10 8.5 9
-
 Cylindrocarpon didymum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.3.10 6 7.5 40°C for 15 min: stable between Cylindrocarpon didymum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.10 FAD
-
 Cylindrocarpon didymum