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Literature summary extracted from

  • Meskys, R.; Harris, R.J.; Casaite, V.; Basran, J.; Scrutton, N.S.
    Organization of the genes involved in dimethylglycine and sarcosine degradation in Arthrobacter spp. Implications for glycine betaine catabolism (2001), Eur. J. Biochem., 268, 3390-3398.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.10 expression in Escherichia coli Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.10 2
-
 N,N-dimethylglycine
-
 Arthrobacter globiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.10 88000
-
 x * 88000, SDS-PAGE Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.10 N,N-dimethylglycine + H2O + O2 Arthrobacter globiformis involved in tetrahydrofolate-dependent assimilation of methyl groups sarcosine + formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.10 Arthrobacter globiformis Q9AGP8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.10 to homogeneity, recombinant enzyme Arthrobacter globiformis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.10 cell culture
-
 Arthrobacter globiformis
-
1.5.3.10 hepatocyte
-
 Arthrobacter globiformis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.10 N,N-dimethylglycine + H2O + O2 involved in tetrahydrofolate-dependent assimilation of methyl groups Arthrobacter globiformis sarcosine + formaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.10 ? x * 88000, SDS-PAGE Arthrobacter globiformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.10 14.3
-
 N,N-dimethylglycine
-
 Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.10 8 10
-
 Arthrobacter globiformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.10 flavin 8alpha-N1-histidyl flavin Arthrobacter globiformis