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Literature summary extracted from

  • Gorlatov, S.N.; Stadtman, T.C.
    Human selenium-dependent thioredoxin reductase from HeLa cells: properties of forms with differing heparin affinities (1999), Arch. Biochem. Biophys., 369, 133-142.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.9 phosphate under growth conditions Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.9 cytosol isoform TrxR1 Homo sapiens 5829
-
1.8.1.9 mitochondrion isoform TrxR2 Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.9 selenocysteine heparin affinity depends on the selenium content, binding can be induced by reduction of the enzyme with NADPH or tris-(2-carboxyethyl)phosphine Homo sapiens
1.8.1.9 selenocysteine contains selenocysteine as part of the redox active selenosulfide in the active center Homo sapiens
1.8.1.9 selenocysteine isoform 1 contains 1 selenium per subunit, the mitochondrial isoform 2 contains 0.5 selenium per subunit Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 54800
-
 2 * 54800, HeLa cell enzyme, MALDI Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Homo sapiens
-
 HeLa cells
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.8.1.9 the reduced enzyme is very labile to O2, decrease of selenium content and activity after exposure to air, protection by NADPH, NADP+ and tris-(2-carboxyethyl)phosphine Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 heparin affinity depends on the selenium content, binding can be induced by reduction of the enzyme with NADPH or tris-(2-carboxyethyl)phosphine Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.9 thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ conformational change upon reduction wth NADPH or tris-(2-carboxyethyl)phosphine Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.9 HeLa cell high and low affinity form with respect to heparin Homo sapiens
-
1.8.1.9 HeLa cell heparin affinity depends on the selenium content Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 thioredoxin + NADP+ coupled assay with DTNB Homo sapiens thioredoxin disulfide + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 dimer 2 * 54800, HeLa cell enzyme, MALDI Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 TrxR
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Homo sapiens