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Literature summary extracted from

  • Reynolds, C.M.; Poole, L.B.
    Attachment of the N-terminal domain of Salmonella typhimurium AhpF to Escherichia coli thioredoxin reductase confers AhpC reductase activity but does not affect thioredoxin reductase activity (2000), Biochemistry, 39, 8859-8869.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 overexpression of wild-type and mutant enzymes from plasmids Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.9 additional information trxB gene is combined to chimeric enzyme NT-TrR by exchanging the N-terminus of Escherichia coli with the N-terminus of Salmonella typhimurium AhpF gene protein, which encodes a protein with about 35% homology in the N-terminal region, 2 other mutants are constructed in the same way but with double mutation C129S/C132S and C342S/C345S, the first in the Escherichia coli part and the latter in the Salmonella part of the chimeric protein, activity corressponding to organism wild-type giving the N-terminal part, except for C342S/C345S chimeric mutant who has no activity Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 additional information
-
 wild-type and chimeric mutants with and without amino acid exchanges Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 thioredoxin + NADP+ coupled assay with DTNB Escherichia coli thioredoxin disulfide + NADPH
-
 ?
1.8.1.9 thioredoxin + NADP+ coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin Escherichia coli thioredoxin disulfide + NADPH
-
 ?
1.8.1.9 thioredoxin + NADP+ NADH in the standard assay, wild-type and chimeric mutants with and without amino acid exchanges Escherichia coli thioredoxin disulfide + NADPH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 TrR
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 additional information
-
 additional information wild-type and recombinant chimeric mutants in a coupled assay Escherichia coli
1.8.1.9 50.3
-
 DTNB chimeric enzyme mutant, partly from Salmonella typhimurium AhpF protein Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD wild-type and chimeric mutants partly from Salmonella typhimurium AhpF protein Escherichia coli
1.8.1.9 NADH wild-type and mutants Escherichia coli
1.8.1.9 NADPH wild-type and mutants Escherichia coli