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Literature summary extracted from

  • Compagnone, D.; Federici, G.; Scarciglia, L.; Palleschi, G.
    Amperometric glutathione electrodes (1993), Biosens. Bioelectron., 8, 257-263.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.8.3.3 analysis development of amperometric glutathione electrodes with immobilized enzyme for detection of H2O2 Penicillium sp.

General Stability

EC Number General Stability Organism
1.8.3.3 after 50 measurements with enzyme immobilized on microelectrode sensor loss of 50% activity Penicillium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.3.3 glutathione + O2 Penicillium sp. measurement of H2O2 in vivo via microelectrodes with immobilized enzyme glutathione disulfide + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.3 Penicillium sp.
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-
-

Storage Stability

EC Number Storage Stability Organism
1.8.3.3 4°C, pH 6.5, phosphate buffer, immobilized enzyme on microelectrode sensor, loss of 20% activity, 60 days Penicillium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.3 2-mercaptoethanol + O2 19% of the activity with glutathione Penicillium sp. mercaptoethanol disulfide
-
 ?
1.8.3.3 D-cysteine + O2 low activity Penicillium sp. D-cystine
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 ?
1.8.3.3 dithiothreitol + O2 38% of the activity with glutathione Penicillium sp. ?
-
 ?
1.8.3.3 glutathione + O2 enzyme immobilized on microsensor Penicillium sp. glutathione disulfide + H2O2
-
 ?
1.8.3.3 glutathione + O2 measurement of H2O2 in vivo via microelectrodes with immobilized enzyme Penicillium sp. glutathione disulfide + H2O2
-
 ?
1.8.3.3 L-cysteine + O2 low activity Penicillium sp. L-cystine
-
 ?
1.8.3.3 L-cysteine ethyl ester + O2 low activity Penicillium sp. L-cystine diethyl ester
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 ?
1.8.3.3 additional information D-penicilamine and coenzyme A are no substrates Penicillium sp. ?
-
 ?
1.8.3.3 N-acetyl-L-cysteine + O2 low activity Penicillium sp. N,N'-diacetyl-L-cystine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.3.3 25
-
 assay with immobilized enzyme on microsensor Penicillium sp.