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Literature summary extracted from

  • Fabianek, R.A.; Hennecke, H.; Thony-Meyer, L.
    Periplasmic protein thiol:disulfide oxidoreductases of Escherichia coli (2000), FEMS Microbiol. Rev., 24, 303-316.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.8.4.2 medicine DsbA protein is a target for treatment of pathogenic bacteria Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.2 additional information DsbA and DsbB insertion mutants are sensitive to dithiothreitol and benzylpenicillin or Cd2+, Hg2+ and Zn2+, pleitropic phenotype Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.2 membrane outer cytoplasmic membrane, protein termini localised in the periplasm Escherichia coli 16020
-
1.8.4.2 periplasm DsbA protein soluble, DsbB protein is membrane intrinsic Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.2 20000
-
 DsbB gene product Escherichia coli
1.8.4.2 21100
-
 DsbA gene product Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.2 GSH + protein disulfide Escherichia coli disulfide bonding step in folding pathway of many periplasmic and outer membrane proteins with structural disulfide bonds GSSG + protein-dithiol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.2 Dickeya chrysanthemi
-
 DsbA homologous
-
1.8.4.2 Dickeya chrysanthemi DsbA homologous
-
 DsbA homologous
-
1.8.4.2 Escherichia coli
-
 in periplasm: DsbA and DsbB enzymes
-
1.8.4.2 Escherichia coli
-
 different forms of protein thiol:disulfide oxidoreductases with either oxidizing, gene DsbA, reducing, gene DsbB, or isomerising, gene DsbC and DsbD, activity exist in Escherichia coli
-
1.8.4.2 Haemophilus influenzae
-
 DsbA homologous
-
1.8.4.2 Haemophilus influenzae DsbA homologous
-
 DsbA homologous
-
1.8.4.2 Klebsiella sp.
-
 DsbA homologous
-
1.8.4.2 Klebsiella sp. DsbA homologous
-
 DsbA homologous
-
1.8.4.2 Legionella pneumophila P50024 DsbA homologous
-
1.8.4.2 Legionella pneumophila DsbA homologous P50024 DsbA homologous
-
1.8.4.2 Lelliottia amnigena Q9XDP0 DsbA homologous
-
1.8.4.2 Lelliottia amnigena DsbA homologous Q9XDP0 DsbA homologous
-
1.8.4.2 Pectobacterium carotovorum
-
 DsbA homologous
-
1.8.4.2 Pectobacterium carotovorum DsbA homologous
-
 DsbA homologous
-
1.8.4.2 Pseudomonas aeruginosa P0C2B2 DsbA homologous
-
1.8.4.2 Pseudomonas aeruginosa DsbA homologous P0C2B2 DsbA homologous
-
1.8.4.2 Pseudomonas syringae O52376 DsbA homologous
-
1.8.4.2 Pseudomonas syringae DsbA homologous O52376 DsbA homologous
-
1.8.4.2 Salmonella enterica subsp. enterica serovar Enteritidis
-
 DsbA homologous
-
1.8.4.2 Salmonella enterica subsp. enterica serovar Enteritidis DsbA homologous
-
 DsbA homologous
-
1.8.4.2 Salmonella enterica subsp. enterica serovar Typhimurium
-
 DsbA homologous
-
1.8.4.2 Salmonella enterica subsp. enterica serovar Typhimurium DsbA homologous
-
 DsbA homologous
-
1.8.4.2 Shigella flexneri
-
 DsbA homologous
-
1.8.4.2 Shigella flexneri DsbA homologous
-
 DsbA homologous
-
1.8.4.2 Vibrio cholerae serotype O1
-
 DsbA homologous
-
1.8.4.2 Vibrio cholerae serotype O1 DsbA homologous
-
 DsbA homologous
-
1.8.4.2 Yersinia pestis
-
 DsbA homologous
-
1.8.4.2 Yersinia pestis DsbA homologous
-
 DsbA homologous
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.4.2 2 glutathione + protein-disulfide = glutathione-disulfide + protein-dithiol C-X-X-C motif in active site for disulfide bonding, either oxidizing, reducing and isomerising activity Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.2 GSH + protein disulfide very rapid disulfide interchange reaction Escherichia coli GSSG + protein-dithiol
-
 r
1.8.4.2 GSH + protein disulfide disulfide bonding step in folding pathway of many periplasmic and outer membrane proteins with structural disulfide bonds Escherichia coli GSSG + protein-dithiol
-
 r
1.8.4.2 additional information DsbB protein re-oxidizes the reduced DsbA protein Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.2 monomer 1 * 21100 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.8.4.2 More DsbA protein family including periplasmic protein disulfide: thiol oxidoreductases with high homology in amino acid sequences Escherichia coli