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Literature summary extracted from

  • Morin, J.E.; Carmichael, C.F.; Dixon, J.E.
    Characterization, kinetics and comparative properties of thiol:protein disulfide oxidoreductase (1978), Arch. Biochem. Biophys., 189, 354-363.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.4.2 EDTA stabilizes during purification Bos taurus
1.8.4.2 GSH, 10 mM, enhances heat stability Bos taurus
1.8.4.2 insulin, 0.087 mM, decreases heat stability Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.4.2 Ca2+ slight Bos taurus
1.8.4.2 Cu2+
-
 Bos taurus
1.8.4.2 deoxycholate
-
 Bos taurus
1.8.4.2 Hg2+
-
 Bos taurus
1.8.4.2 Mg2+ slight inhibition Bos taurus
1.8.4.2 Zn2+ slight inhibition Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.2 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.8.4.2 glycoprotein
-
 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.2
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.2 2-mercaptoethanol + protein disulfide
-
 Bos taurus 2-mercaptoethanol disulfide + protein-dithiol
-
 ?
1.8.4.2 dithiothreitol + protein disulfide
-
 Bos taurus ?
-
 ?
1.8.4.2 GSH + insulin
-
 Bos taurus GSSG + reduced insulin chain A and B
-
 ?
1.8.4.2 GSH + oxytoxin
-
 Bos taurus ?
-
 ?
1.8.4.2 GSH + protein disulfide
-
 Bos taurus GSSG + protein-dithiol
-
 ?
1.8.4.2 GSH + vasopressin
-
 Bos taurus ?
-
 ?
1.8.4.2 GSH + vasotocin
-
 Bos taurus ?
-
 ?
1.8.4.2 GSSG + reduced ribonuclease
-
 Bos taurus GSH + oxidized ribonuclease
-
 ?
1.8.4.2 L-cysteine + protein disulfide
-
 Bos taurus cystine + protein-dithiol
-
 ?
1.8.4.2 additional information enzyme also catalyzes reactivation and folding of protein containing incorrectly paired disulfide bond, e.g.: scrambled ribonuclease Bos taurus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.4.2 60
-
 30 min, 50% loss of activity Bos taurus
1.8.4.2 65
-
 30 min, 90% loss of activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.2 7.5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.4.2 6.2 10.2 pH 6.2: about 10% of activity maximum, pH 10.2: about 20% of activity maximum Bos taurus