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Literature summary extracted from
- Purification and characterization of trypanothione reductase from Crithidia fasciculata, a newly discovered member of the family of disulfide-containing flavoprotein reductases (1986), Biochemistry, 25, 3519-3526.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.12 | additional information | activity is dependent on a redox-active cysteine at the active centre | Crithidia fasciculata |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.12 | iodoacetamide | inhibition only of the enzyme with reduced cysteine residues in the catalytic center by alkylation | Crithidia fasciculata |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.12 | 0.005 | 0.007 | NADPH | - |
Crithidia fasciculata | |
| 1.8.1.12 | 0.053 | - |
trypanothione disulfide | - |
Crithidia fasciculata | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.12 | 53800 | - |
1 * 53800, SDS-PAGE | Crithidia fasciculata |
| 1.8.1.12 | 110000 | - |
enzyme cross-linked with dimethyl suberimidate, SDS-PAGE | Crithidia fasciculata |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.12 | trypanothione disulfide + NADPH | Crithidia fasciculata | - |
trypanothione + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.12 | Crithidia fasciculata | - |
insect-parasitic trypanosomatid | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.12 | - |
Crithidia fasciculata |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.12 | 284 | - |
purified enzyme | Crithidia fasciculata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.12 | trypanothione disulfide + NADPH | - |
Crithidia fasciculata | trypanothione + NADP+ | - |
? | |
| 1.8.1.12 | trypanothione disulfide + NADPH | highly specific for | Crithidia fasciculata | trypanothione + NADP+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.12 | dimer | 1 * 53800, SDS-PAGE | Crithidia fasciculata |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.12 | 517 | - |
trypanothione disulfide | - |
Crithidia fasciculata | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.12 | 7.5 | 8 | trypanothione disulfide + NADPH | Crithidia fasciculata |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.12 | FAD | - |
Crithidia fasciculata | |
| 1.8.1.12 | NADPH | - |
Crithidia fasciculata | |
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