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Literature summary extracted from

  • Krauth-Siegel, R.L.; Enders, B.; Henderson, G.B.; Fairlamb, A.H.; Schirmer, R.H.
    Trypanothione reductase from Trypanosoma cruzi. Purification and characterization of the crystalline enzyme (1987), Eur. J. Biochem., 164, 123-128.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.12 additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma cruzi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.12 hanging drop method in presence of 2 M (NH4)2SO4 at 4°C Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.12 iodoacetamide inhibition only of the enzyme with reduced cysteine residues in the catalytic center by alkylation Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.12 additional information
-
 additional information Km dependent on assay conditions Trypanosoma cruzi
1.8.1.12 0.005 0.007 NADPH
-
 Trypanosoma cruzi
1.8.1.12 0.022 0.275 glutathionylspermidine disulfide
-
 Trypanosoma cruzi
1.8.1.12 0.045
-
 trypanothione disulfide
-
 Trypanosoma cruzi
1.8.1.12 0.275
-
 glutathionylspermidine disulfide
-
 Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.12 50000
-
 2 * 50000, SDS-PAGE Trypanosoma cruzi
1.8.1.12 100000
-
 gel filtration Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.12 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.12
-
 Trypanosoma cruzi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.12 284
-
 purified enzyme Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.12 glutathionylspermidine disulfide + NADPH
-
 Trypanosoma cruzi 2-glutathionylspermidine + NADP+
-
 r
1.8.1.12 trypanothione disulfide + NADPH
-
 Trypanosoma cruzi trypanothione + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.1.12 dimer 2 * 50000, SDS-PAGE Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.12 237
-
 trypanothione disulfide dependent on assay conditions Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.12 FAD
-
 Trypanosoma cruzi
1.8.1.12 NADH low activity with NADH Trypanosoma cruzi
1.8.1.12 NADPH enzyme also utilizes NADH, but less efficiently Trypanosoma cruzi