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Literature summary extracted from

  • Stoll, V.S.; Simpson, S.J.; Krauth-Siegel, R.L.; Walsh, C.T.; Pai, E.F.
    Glutathione reductase turned into trypanothione reductase: structural analysis of an engineered change in substrate specificity (1997), Biochemistry, 36, 6437-6447.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.7 cysteine redox active disulfide at the catalytic site Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.7 mutant enzyme glutathione reductase-trypanothine reductase, expression in Escherichia coli Homo sapiens
1.8.1.12 mutant enzyme glutathione reductase-trypanothine reductase, expression in Escherichia coli Homo sapiens
1.8.1.12 overexpression in Escherichia coli Trypanosoma cruzi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.7 substrate-binding and structure analysis Homo sapiens
1.8.1.12 recombinant glutathione-trypanothione reductase-like enzyme, hanging-drop vapour diffusion method, 0.1 M potasssium phosphate, pH 8.0 Homo sapiens
1.8.1.12 substrate-binding and structure analysis Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.7 A34E/R37W glutathione reductase mutant, activity switches to trypanothione reductase, termed GRTR, 700fold more activity with trypanothione than with glutathione Homo sapiens
1.8.1.12 A34E/R37W glutathione reductase mutant, activity switches to trypanothione reductase, termed GRTR, 700fold more activity with trypanothione disulfide than with glutathione Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.065
-
 GSSG
-
 Homo sapiens
1.8.1.7 0.5
-
 trypanothione recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens
1.8.1.12 0.018
-
 trypanothione disulfide
-
 Trypanosoma cruzi
1.8.1.12 0.5
-
 trypanothione disulfide recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Homo sapiens P00390
-
-
1.8.1.12 Homo sapiens
-
-
-
1.8.1.12 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7 recombinant mutant from Escherichia coli Homo sapiens
1.8.1.12 recombinant mutant from Escherichia coli Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.7 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ member of pyridine-nucleotide disulfide oxidoreductase family of flavoenzymes Homo sapiens
a
1.8.1.7 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ recombinant glutathione-trypanothione reductase-like enzyme, substrate binding, three dimensional structure, complex formation Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 GSSG + NADPH
-
 Homo sapiens glutathione + NADP+
-
 ?
1.8.1.7 trypanothione + NADPH recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens reduced trypanothione + NADP+
-
 ?
1.8.1.12 trypanothione disulfide + NADPH
-
 Trypanosoma cruzi trypanothione + NADP+
-
 ?
1.8.1.12 trypanothione disulfide + NADPH recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens trypanothione + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 GR
-
 Homo sapiens
1.8.1.12 TR
-
 Homo sapiens
1.8.1.12 TR
-
 Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.7 20.8
-
 trypanothione recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens
1.8.1.7 210
-
 GSSG
-
 Homo sapiens
1.8.1.12 20.8
-
 trypanothione disulfide recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 6.5
-
 recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens
1.8.1.12 6.5
-
 recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens
1.8.1.12 7.25 7.75
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 FAD
-
 Homo sapiens
1.8.1.7 NADPH
-
 Homo sapiens
1.8.1.12 FAD
-
 Homo sapiens
1.8.1.12 FAD
-
 Trypanosoma cruzi
1.8.1.12 NADPH
-
 Homo sapiens
1.8.1.12 NADPH
-
 Trypanosoma cruzi