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Literature summary extracted from

  • Krauth-Siegel, R.L.; Jockers-Scheruebl, M.C.; Becker, K.; Schirmer, R.H.
    NADPH-dependent disulphide reductases (1989), Biochem. Soc. Trans., 17, 315-317.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.7 cysteine redox active disulfide at the catalytic site Homo sapiens
1.8.1.12 additional information activity is dependent on a redox-active cysteine at the active centre Crithidia fasciculata
1.8.1.12 additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma cruzi
1.8.1.12 additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma congolense

Application

EC Number Application Comment Organism
1.8.1.7 medicine mimicking of favism by inhibiting the enzyme with specific inhibitors to protect against malaria Homo sapiens
1.8.1.7 medicine potential target of antimalarial and cytostatic agents Homo sapiens
1.8.1.12 medicine potential target for drug design against trypanosomiasis Crithidia fasciculata
1.8.1.12 medicine potential target for drug design against trypanosomiasis Trypanosoma cruzi
1.8.1.12 medicine potential target for drug design against trypanosomiasis Trypanosoma congolense

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.7
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 carmustine irreversible inhibito of enzyme in reduced state Homo sapiens
1.8.1.7 NADPH slow inactivation in vitro due to inter- or intramolecular disulfide formation Homo sapiens
1.8.1.12 nifurtimox weak inhibitor Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 100000
-
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Homo sapiens
-
-
-
1.8.1.12 Crithidia fasciculata
-
 insect-parasitic trypanosomatid
-
1.8.1.12 Trypanosoma congolense
-
-
-
1.8.1.12 Trypanosoma cruzi
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.7 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ mechanism Homo sapiens
a
1.8.1.7 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ member of pyridine-nucleotide disulfide oxidoreductase family of flavoenzymes Homo sapiens
a
1.8.1.7 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ catalytic cycle Homo sapiens
a
1.8.1.7 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ substrate and cofactor binding site, three-dimensional structure Homo sapiens
a
1.8.1.12 trypanothione + NADP+ = trypanothione disulfide + NADPH + H+ mechanistic scheme for NAD(P)H:disulfide oxidoreductases Crithidia fasciculata
a
1.8.1.12 trypanothione + NADP+ = trypanothione disulfide + NADPH + H+ mechanistic scheme for NAD(P)H:disulfide oxidoreductases Trypanosoma cruzi
a
1.8.1.12 trypanothione + NADP+ = trypanothione disulfide + NADPH + H+ mechanistic scheme for NAD(P)H:disulfide oxidoreductases Trypanosoma congolense
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 GSSG + NADPH
-
 Homo sapiens glutathione + NADP+
-
 r
1.8.1.12 trypanothione disulfide + NADPH
-
 Crithidia fasciculata trypanothione + NADP+
-
 r
1.8.1.12 trypanothione disulfide + NADPH
-
 Trypanosoma cruzi trypanothione + NADP+
-
 r
1.8.1.12 trypanothione disulfide + NADPH
-
 Trypanosoma congolense trypanothione + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.1.12 dimer
-
 Crithidia fasciculata
1.8.1.12 dimer
-
 Trypanosoma cruzi
1.8.1.12 dimer
-
 Trypanosoma congolense

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 FAD FAD enzyme Homo sapiens
1.8.1.7 additional information as malaria brings riboflavin deficiency, in effection regions a large portion of enzymes miss FAD, but are as well active Homo sapiens
1.8.1.7 NADPH can utilize both NADPH and NADH, more active with NADPH Homo sapiens
1.8.1.12 FAD
-
 Crithidia fasciculata
1.8.1.12 FAD
-
 Trypanosoma cruzi
1.8.1.12 FAD
-
 Trypanosoma congolense
1.8.1.12 NADPH
-
 Crithidia fasciculata
1.8.1.12 NADPH
-
 Trypanosoma cruzi
1.8.1.12 NADPH
-
 Trypanosoma congolense

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.12 0.15
-
 nifurtimox
-
 Trypanosoma cruzi