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Literature summary extracted from

  • Sugio, T.; Matsugi, S.; Tanaka, T.; Fujita, M.; Tano, T.
    Purification and some properties of glutathione reductase from iron-oxidizing bacterium Thiobacillus ferrooxidans (1995), Biosci. Biotechnol. Biochem., 59, 1568-1570.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.026
-
 NADPH
-
 Acidithiobacillus ferrooxidans
1.8.1.7 0.125
-
 NADH
-
 Acidithiobacillus ferrooxidans
1.8.1.7 0.3
-
 GSSG
-
 Acidithiobacillus ferrooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 52000
-
 2 * 52000, SDS-PAGE Acidithiobacillus ferrooxidans
1.8.1.7 100000
-
 gel filtration and PAGE Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Acidithiobacillus ferrooxidans
-
 iron-grown, AP19-3
-
1.8.1.7 Acidithiobacillus ferrooxidans AP19-3
-
 iron-grown, AP19-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 350
-
 purified enzyme Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADH + H+
-
 Acidithiobacillus ferrooxidans glutathione + NAD+
-
 ?
1.8.1.7 glutathione disulfide + NADH + H+
-
 Acidithiobacillus ferrooxidans AP19-3 glutathione + NAD+
-
 ?
1.8.1.7 GSSG + NADPH
-
 Acidithiobacillus ferrooxidans glutathione + NADP+
-
 ?
1.8.1.7 GSSG + NADPH
-
 Acidithiobacillus ferrooxidans AP19-3 glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 dimer 2 * 52000, SDS-PAGE Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 40
-
-
 Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 6.5
-
 NADPH + GSSG Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADPH
-
 Acidithiobacillus ferrooxidans