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Literature summary extracted from

  • Jiang, F.; Mannervik, B.
    Optimized heterologous expression of glutathione reductase from cyanobacterium Anabaena PCC 7120 and characterization of the recombinant protein (1999), Protein Expr. Purif., 15, 92-98.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.7 expression in Escherichia coli of a His-tagged high-level expression mutant Anabaena sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.7 additional information introduction of silent random mutations in 5'-region to generate a His-tagged high-level expression clone Anabaena sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.16
-
 GSSG recombinant enzyme Anabaena sp.
1.8.1.7 0.24
-
 NADPH recombinant enzyme Anabaena sp.
1.8.1.7 1.12
-
 NADH recombinant enzyme Anabaena sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 GSSG + NADPH Anabaena sp. high intracellular concentration of glutathione is important for functionality of thiol groups of cellular proteins glutathione + NADP+
-
 ir
1.8.1.7 GSSG + NADPH Anabaena sp. serves in detoxification glutathione + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Anabaena sp.
-
 PCC 7120, cyanobacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7 via high-level expression of recombinant enzyme in Escherichia coli Anabaena sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 246
-
 recombinant purified enzyme Anabaena sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADH + H+
-
 Anabaena sp. glutathione + NAD+
-
 ?
1.8.1.7 GSSG + NADPH
-
 Anabaena sp. glutathione + NADP+
-
 ir
1.8.1.7 GSSG + NADPH high intracellular concentration of glutathione is important for functionality of thiol groups of cellular proteins Anabaena sp. glutathione + NADP+
-
 ir
1.8.1.7 GSSG + NADPH serves in detoxification Anabaena sp. glutathione + NADP+
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 GR
-
 Anabaena sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.7 38
-
 NADH recombinant enzyme Anabaena sp.
1.8.1.7 367
-
 NADPH recombinant enzyme Anabaena sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7
-
 NADPH + GSSG Anabaena sp.
1.8.1.7 8
-
 NADH + GSSG Anabaena sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADH low activity with NADH Anabaena sp.
1.8.1.7 NADPH can utilize both NADPH and NADH, more active with NADPH Anabaena sp.