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Literature summary extracted from

  • Garcia-Alfonso, C.; Martinez-Galisteo, E.; Llobell, A.; Barcena, J.A.; Lopez-Barea, J.
    Horse-liver glutathione reductase: purification and characterization (1993), Int. J. Biochem., 25, 61-68.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 KCl
-
 Equus caballus
1.8.1.7 KI
-
 Equus caballus
1.8.1.7 KNO3
-
 Equus caballus
1.8.1.7 Na2S2O3
-
 Equus caballus
1.8.1.7 NaCl
-
 Equus caballus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 additional information
-
 additional information different isozymes, temperature-dependent, homo-glutathione Equus caballus
1.8.1.7 0.009
-
 NADPH
-
 Equus caballus
1.8.1.7 0.059
-
 GSSG
-
 Equus caballus
1.8.1.7 0.56
-
 ferricyanide
-
 Equus caballus
1.8.1.7 0.68
-
 NADH
-
 Equus caballus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.7 KBr below 0.1 M Equus caballus
1.8.1.7 KH2PO4 below 0.1 M Equus caballus
1.8.1.7 NaF below 0.1 M Equus caballus
1.8.1.7 NH4Cl below 0.1 M Equus caballus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 107000
-
 native PAGE Equus caballus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Equus caballus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Equus caballus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 liver
-
 Equus caballus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 248
-
-
 Equus caballus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 ferricyanide + NADPH + H+
-
 Equus caballus ferrocyanide + NADP+
-
 r
1.8.1.7 glutathione disulfide + NADH + H+
-
 Equus caballus glutathione + NAD+
-
 ?
1.8.1.7 GSSG + NADPH ferricyanide and 2,6-dichlorophenolindophenol as electron acceptors Equus caballus glutathione + NADP+
-
 r
1.8.1.7 GSSG + NADPH mixed disulfide between coenzyme A and glutathione Equus caballus glutathione + NADP+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 6.5
-
 NADH + glutathione Equus caballus
1.8.1.7 7
-
 NADPH + GSSG Equus caballus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 deamino-NADPH 69% of the activity with NADPH Equus caballus
1.8.1.7 ferricyanide
-
 Equus caballus
1.8.1.7 flavin flavoprotein Equus caballus
1.8.1.7 additional information total lack of activity with 3-acetylpyridine NADH Equus caballus
1.8.1.7 NADH 8.5% of the activity with NADPH Equus caballus
1.8.1.7 NADPH
-
 Equus caballus