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Literature summary extracted from
- Glutathione reductase in the sea urchin egg. I. Purification and general properties (1974), Biochim. Biophys. Acta, 350, 141-150.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | Iodide | - |
Hemicentrotus pulcherrimus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.7 | 0.00078 | - |
FAD | - |
Hemicentrotus pulcherrimus | |
| 1.8.1.7 | 0.005 | - |
NADPH | - |
Hemicentrotus pulcherrimus | |
| 1.8.1.7 | 0.101 | - |
GSSG | - |
Hemicentrotus pulcherrimus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | phosphate | optimum concentration of potassium phosphate: 0.1 M | Hemicentrotus pulcherrimus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 52000 | - |
2 * 52000, SDS-PAGE | Hemicentrotus pulcherrimus |
| 1.8.1.7 | 102000 | - |
gel filtration | Hemicentrotus pulcherrimus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | Hemicentrotus pulcherrimus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | - |
Hemicentrotus pulcherrimus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.8.1.7 | 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ | mechanism | Hemicentrotus pulcherrimus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | egg | - |
Hemicentrotus pulcherrimus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 320 | - |
purified enzyme | Hemicentrotus pulcherrimus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.8.1.7 | -28°C, 50 mM phosphate buffer, a few months | Hemicentrotus pulcherrimus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.7 | GSSG + NADPH | - |
Hemicentrotus pulcherrimus | glutathione + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | dimer | 2 * 52000, SDS-PAGE | Hemicentrotus pulcherrimus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 25 | - |
assay at | Hemicentrotus pulcherrimus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 7.2 | 7.3 | - |
Hemicentrotus pulcherrimus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | FAD | FAD enzyme | Hemicentrotus pulcherrimus | |
| 1.8.1.7 | additional information | no activity with FMN | Hemicentrotus pulcherrimus | |
| 1.8.1.7 | NADH | less than 1% activity of that with NADPH | Hemicentrotus pulcherrimus | |
| 1.8.1.7 | NADPH | - |
Hemicentrotus pulcherrimus | |
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