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Literature summary extracted from
- Purification of glutathione reductase from porcine erythrocytes by the use of affinity chromatography on 2,5-ADP-Sepharose 4B and crystallization of the enzyme (1979), Anal. Biochem., 98, 335-340.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | hanging-drop method at 22°C, size of crystals varies with ammonium sulfate concentration | Sus scrofa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.7 | 0.021 | - |
NADPH | - |
Sus scrofa |  |
| 1.8.1.7 | 0.076 | - |
GSSG | - |
Sus scrofa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 59000 | - |
2 * 59000, SDS-PAGE | Sus scrofa |
| 1.8.1.7 | 103000 | - |
analytical ultracentrifugation | Sus scrofa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | - |
Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | erythrocyte | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 221 | - |
purified enzyme | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.7 | GSSG + NADPH | - |
Sus scrofa | glutathione + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | dimer | 2 * 59000, SDS-PAGE | Sus scrofa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 5 | - |
NADH + GSSG | Sus scrofa |
| 1.8.1.7 | 7 | - |
NADPH + GSSG | Sus scrofa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | FAD | 2 mol of FAD per mol of enzyme | Sus scrofa | |
| 1.8.1.7 | FAD | FAD enzyme | Sus scrofa | |
| 1.8.1.7 | NADH | activity relative to NADPH: 0.5% | Sus scrofa | |
| 1.8.1.7 | NADPH | best electron donor | Sus scrofa | |
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