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Literature summary extracted from

  • Montero, S.; De Arriaga, D.; Soler, J.
    Purification and characterization of glutathione reductase from mycelium of Phycomyces blakesleeanus (1988), Biochim. Biophys. Acta, 952, 56-66.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.8.1.7 urea, quite stable to Phycomyces blakesleeanus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 NADH
-
 Phycomyces blakesleeanus
1.8.1.7 NADPH
-
 Phycomyces blakesleeanus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.02
-
 NADPH
-
 Phycomyces blakesleeanus
1.8.1.7 0.13
-
 GSSG
-
 Phycomyces blakesleeanus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 50200
-
 2 * 50200 Phycomyces blakesleeanus
1.8.1.7 100000
-
-
 Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 GSSG + NADPH Phycomyces blakesleeanus
-
 glutathione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Phycomyces blakesleeanus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Phycomyces blakesleeanus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 mycelium
-
 Phycomyces blakesleeanus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 61.2
-
-
 Phycomyces blakesleeanus

Storage Stability

EC Number Storage Stability Organism
1.8.1.7 4°C, pH 7.5, 50 mM sodium phosphate buffer, 1 mM 2-mercaptoethanol, 1 mM EDTA Phycomyces blakesleeanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 GSSG + NADPH
-
 Phycomyces blakesleeanus glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 dimer 2 * 50200 Phycomyces blakesleeanus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.7 42.5
-
 complete loss of activity above 42°C, 10 min in presence of NADPH Phycomyces blakesleeanus
1.8.1.7 55
-
 10 min, 13% loss of activity Phycomyces blakesleeanus
1.8.1.7 65
-
 10 min, complete loss of activity Phycomyces blakesleeanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 6
-
 NADH Phycomyces blakesleeanus
1.8.1.7 7.5
-
 NADPH + GSSG Phycomyces blakesleeanus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 FAD 2 mol of FAD per mol of enzyme Phycomyces blakesleeanus
1.8.1.7 NADPH
-
 Phycomyces blakesleeanus