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Literature summary extracted from
- Glutathione reductase (yeast) (1971), Methods Enzymol., 17, 500-503.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | iodoacetate | in presence but not in absence of reduced coenzyme | Saccharomyces cerevisiae |  |
| 1.8.1.7 | sulfhydryl reagents | in presence but not in absence of reduced coenzyme | Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.7 | 0.0038 | - |
NADPH | - |
Saccharomyces cerevisiae | |
| 1.8.1.7 | 0.055 | - |
GSSG | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 118000 | - |
equilibrium sedimentation | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | - |
Saccharomyces cerevisiae |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | refolding after treatment with 6 M urea, recovering of full activity | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 153 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.8.1.7 | -20°C, pH 6.8, 0.05 M potassium phosphate buffer, indefinitely stable | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.7 | GSSG + NADPH | - |
Saccharomyces cerevisiae | glutathione + NADP+ | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 6.6 | 7.6 | - |
Saccharomyces cerevisiae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 3 | - |
stable | Saccharomyces cerevisiae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | FAD | FAD enzyme | Saccharomyces cerevisiae | |
| 1.8.1.7 | NADPH | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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