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Literature summary extracted from

  • Yanagawa, H.
    Asparagusate dehydrogenase and lipoyl deydrogenase from Asparagus (1979), Methods Enzymol., 62, 172-181.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.1.11 EDTA ion stabilizes the enzyme Asparagus officinalis
1.8.1.11 PO43- stabilizes the enzyme Asparagus officinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.11 arsenite low inhibition Asparagus officinalis
1.8.1.11 asparagusic acid inhibits lipoyl dehydrogenase activity Asparagus officinalis
1.8.1.11 Hg2+ inhibitory Asparagus officinalis
1.8.1.11 iodoacetamide low inhibition Asparagus officinalis
1.8.1.11 additional information inhibitors indicate an involvement of protein disulfide linkage or thiol group in the catalytic site Asparagus officinalis
1.8.1.11 N-ethylmaleimide
-
 Asparagus officinalis
1.8.1.11 NADH above 0.1 mM Asparagus officinalis
1.8.1.11 p-chloromercuribenzoic acid
-
 Asparagus officinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.11 0.8
-
 potassium ferricyanide enzyme II Asparagus officinalis
1.8.1.11 0.9
-
 potassium ferricyanide enzyme I Asparagus officinalis
1.8.1.11 3
-
 lipoic acid
-
 Asparagus officinalis
1.8.1.11 20
-
 asparagusic acid
-
 Asparagus officinalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.11 mitochondrion
-
 Asparagus officinalis 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.11 110000 111000 enzyme I and II, sedimentation equilibrium Asparagus officinalis
1.8.1.11 112000
-
 calculation of amino acid content Asparagus officinalis
1.8.1.11 112000
-
 enzyme I and II, gel filtration Asparagus officinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.11 asparagusate + NADH Asparagus officinalis essential component of pyruvate dehydrogenase complex dihydroasparagusate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.11 Asparagus officinalis
-
 2 asparagusate dehydrogenases with lipoyl dehydrogenase activity
-
1.8.1.11 Asparagus officinalis
-
 etiolated or green, activity levels are higher in etiolated than in green shoots
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.11 gel filtration, ion-exchange, copurification of enzyme I and II Asparagus officinalis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.11 3-mercapto-2-mercaptomethylpropanoate + NAD+ = asparagusate + NADH + H+ exhibits lipoyl dehydrogenase activity Asparagus officinalis
a
1.8.1.11 3-mercapto-2-mercaptomethylpropanoate + NAD+ = asparagusate + NADH + H+ exhibits diaphorase activity Asparagus officinalis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.11 shoot
-
 Asparagus officinalis
-
1.8.1.11 shoot apex around, higher levels than other regions Asparagus officinalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.11 0.215
-
 enzyme/fraction II towards asparagusic acid Asparagus officinalis
1.8.1.11 0.425
-
 enzyme/fraction I towards asparagusic acid Asparagus officinalis
1.8.1.11 0.5464
-
 enzyme/fraction II towards lipoic acid Asparagus officinalis
1.8.1.11 1.082
-
 enzyme/fraction I towards lipoic acid Asparagus officinalis

Storage Stability

EC Number Storage Stability Organism
1.8.1.11 -80°C, 67 mM sodium phosphate, pH 7.0, no loss of activity for at least 1 month Asparagus officinalis
1.8.1.11 4°C, 67 mM sodium phosphate, pH 7.0, very unstable Asparagus officinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.11 2 ferricyanide + NADH
-
 Asparagus officinalis 2 ferrocyanide + NAD+ + H+
-
 ?
1.8.1.11 asparagusate + NADH essential component of pyruvate dehydrogenase complex Asparagus officinalis dihydroasparagusate + NAD+
-
 r
1.8.1.11 asparagusic acid + NADH reverse reaction very slow Asparagus officinalis dihydroasparagusate + NAD+
-
 r
1.8.1.11 lipoic acid + NADH reverse reaction very slow Asparagus officinalis dihydrolipoic acid + NAD+
-
 r
1.8.1.11 NADH + H+ + potassium ferricyanide
-
 Asparagus officinalis NAD+ + potassium ferrocyanide
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.11 25
-
 assay at Asparagus officinalis
1.8.1.11 40 45
-
 Asparagus officinalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.11 50
-
 5 min, activity completely retained Asparagus officinalis
1.8.1.11 50
-
 above, gradual loss of lipoyl dehydrogenase activity, complete loss at 90°C Asparagus officinalis
1.8.1.11 60 70 5 min, 1.4-fold activation of asparagusate dehydrogenase activity Asparagus officinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.11 5.25
-
 for K3Fe(CN)6 reduction Asparagus officinalis
1.8.1.11 5.9
-
-
 Asparagus officinalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.11 FAD flavoprotein, approximately 1 mol of FAD per mol of protein Asparagus officinalis
1.8.1.11 NAD+ up to 0.2 mM, stimulates the reduction activity if NADH concentration is 0.2 mM Asparagus officinalis
1.8.1.11 NADH no replacement of NADH by NADPH Asparagus officinalis
1.8.1.11 NADH 0.1 mM optimal concentration for maximal activity Asparagus officinalis