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Literature summary extracted from

  • Battelli, M.G.
    Enzymic conversion of rat liver xanthine oxidase from dehydrogenase (D form) to oxidase (O form) (1980), FEBS Lett., 113, 47-51.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.7 Rattus norvegicus
-
 Wistar
-
1.8.4.7 Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.7 ammonium sulfate precipitation, gel filtration, ion-exchange Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.4.7 [xanthine dehydrogenase] + glutathione disulfide = [xanthine oxidase] + 2 glutathione thioltransferase activity with cysteine as substrate. Mainly D-form of enzyme exists Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.7 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.7 additional information
-
 3750 U/mg, 1 unit is defined as the amount of the enzyme producing a 1% reduction of the ration NADH formed/uric acid formed, corrected for non-enzymic conversion Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.8.4.7 -25°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.7 [xanthine dehydrogenase] + oxidized glutathione reverse conversion possible by dithioerythritol, not obtained in presence of GSH Rattus norvegicus [xanthine oxidase] + glutathione
-
 r
1.8.4.7 [xanthine dehydrogenase] + oxidized glutathione reverse conversion possible by dithioerythritol, not obtained in presence of GSH Rattus norvegicus Wistar [xanthine oxidase] + glutathione
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.7 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.7 7 8.5
-
 Rattus norvegicus
1.8.4.7 8.1
-
 assay at Rattus norvegicus