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Literature summary extracted from

  • Battelli, M.G.; Lorenzoni, E.
    Purification and properties of a new glutathione-dependent thiol:disulphide oxidoreductase from rat liver (1982), Biochem. J., 207, 133-138.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.4.7 additional information insensitive against 1 mM CuSO4 and 1 mM N-ethylmaleimide at 37°C for 30 min Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.7 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.7 40000
-
 1 * 40000, SDS-PAGE Rattus norvegicus
1.8.4.7 43000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O Rattus sp. conversion of xanthine dehydrogenase to the oxidase type by thiol-disulfide oxidoreductase, thiol reagents or oxidized glutathione urate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.7 Rattus norvegicus
-
 newborn and adults
-
1.17.1.4 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.7 ion-exchange, gel filtration Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.4.7 [xanthine dehydrogenase] + glutathione disulfide = [xanthine oxidase] + 2 glutathione thioltransferase, GSH:insulin transhydrogenase and ricin-disulphide-bond reductase activity Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.7 brain
-
 Rattus norvegicus
-
1.8.4.7 heart
-
 Rattus norvegicus
-
1.8.4.7 kidney
-
 Rattus norvegicus
-
1.8.4.7 liver highest amount of activity Rattus norvegicus
-
1.8.4.7 lung
-
 Rattus norvegicus
-
1.8.4.7 skeletal muscle
-
 Rattus norvegicus
-
1.8.4.7 skin
-
 Rattus norvegicus
-
1.8.4.7 spleen
-
 Rattus norvegicus
-
1.17.1.4 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.7 additional information
-
 372 U/mg, other definition of units Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.7 additional information other disulfide compounds are either inactive or far less active than oxidized glutathione, reduction of disulfide bonds in ricin, thioltransferase activity, GSH:insulin transhydrogenase activity Rattus norvegicus ?
-
 ?
1.8.4.7 [xanthine dehydrogenase] + oxidized glutathione conversion into oxidase by treatment with proteolytic enzymes, storage at -20°C/-25°C or preincubation with either thiol reagents or whole liver homogenate. Can act in the opposite direction in the reduction of the disulfide bond of insulin and ricin Rattus norvegicus [xanthine oxidase] + glutathione
-
 r
1.17.1.4 xanthine + NAD+ + H2O conversion of xanthine dehydrogenase to the oxidase type by thiol-disulfide oxidoreductase, thiol reagents or oxidized glutathione Rattus sp. urate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.7 monomer 1 * 40000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.7 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.7 8.1
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 NAD+
-
 Rattus sp.