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Literature summary extracted from

  • Hartmann, G.C.; Klein, A.R.; Linder, M.; Thauer, R.K.
    Purification, properties and primary structure of H2-forming N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanococcus thermolithotrophicus (1996), Arch. Microbiol., 165, 187-193.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.98.2
-
 Methanothermococcus thermolithotrophicus

General Stability

EC Number General Stability Organism
1.12.98.2 50% loss of activity after freezing and thawing Methanothermococcus thermolithotrophicus
1.12.98.2 the rate of inactivation increases upon dilution and in the presence of O2 and decreases upon the addition of 5 mg/ml albumin Methanothermococcus thermolithotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.98.2 0.06
-
 N5,N10-methylenetetrahydromethanopterin
-
 Methanothermococcus thermolithotrophicus
1.12.98.2 0.15
-
 H2
-
 Methanothermococcus thermolithotrophicus
1.12.98.2 0.85
-
 N5,N10-methenyltetrahydromethanopterin
-
 Methanothermococcus thermolithotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.2 additional information the enzyme does not contain nickel or iron/sulfur clusters Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.2 37992
-
 4 * 37992, calculation from nucleotide sequence Methanothermococcus thermolithotrophicus
1.12.98.2 38038
-
 4 * 38038, MALDI-TOF-MS analysis of peptides obtained by BrCN cleavage Methanothermococcus thermolithotrophicus
1.12.98.2 43000
-
 4 * 43000, SDS-PAGE Methanothermococcus thermolithotrophicus
1.12.98.2 150000
-
 gel filtration Methanothermococcus thermolithotrophicus

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.2 Methanothermobacter thermautotrophicus
-
-
-
1.12.98.2 Methanothermococcus thermolithotrophicus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.98.2 purified enzyme is very labile even under strictly anoxic conditions Methanothermococcus thermolithotrophicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.2
-
 Methanothermococcus thermolithotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.2 440
-
-
 Methanothermococcus thermolithotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.2 N5,N10-methylenetetrahydromethanopterin + H+ r Methanothermobacter thermautotrophicus N5,N10-methenyltetrahydromethanopterin + H2
-
 ?
1.12.98.2 N5,N10-methylenetetrahydromethanopterin + H+ r Methanothermococcus thermolithotrophicus N5,N10-methenyltetrahydromethanopterin + H2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.2 tetramer 4 * 43000, SDS-PAGE Methanothermococcus thermolithotrophicus
1.12.98.2 tetramer 4 * 37992, calculation from nucleotide sequence Methanothermococcus thermolithotrophicus
1.12.98.2 tetramer 4 * 38038, MALDI-TOF-MS analysis of peptides obtained by BrCN cleavage Methanothermococcus thermolithotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.98.2 80
-
-
 Methanothermococcus thermolithotrophicus