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Literature summary extracted from

  • Ji, L.; Becana, M.; Sarath, G.; Shearman, L.; Klucas, R.V.
    Overproduction in Escherichia coli and characterization of a soybean ferric leghemoglobin reductase (1994), Plant Physiol., 106, 203-209.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.6 expression in Escherichia coli Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.6 0.0063
-
 ferrileghemoglobin recombinant enzyme Glycine max
1.6.2.6 0.0092
-
 ferrileghemoglobin native enzyme Glycine max
1.6.2.6 0.046
-
 NADH lipoamide-dependent NADH oxidation Glycine max
1.6.2.6 0.716
-
 Lipoamide lipoamide-dependent NADH oxidation Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.6 NAD(P)H + ferrileghemoglobin Glycine max proposed to be involved in maintaining leghemoglobin in an active form in legume nodules NAD(P)+ + ferroleghemoglobin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.6 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.6 recombinant enzyme, Sepharose 6B, Probond Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.6 0.486
-
 recombinant enzyme, reduction of ferrileghemoglobin Glycine max
1.6.2.6 4.47
-
 recombinant enzyme, reduction of 2,6-dichloroindophenol Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.6 NAD(P)H + ferrileghemoglobin proposed to be involved in maintaining leghemoglobin in an active form in legume nodules Glycine max NAD(P)+ + ferroleghemoglobin
-
 ?
1.6.2.6 NADH + lipoamide recombinant enzyme, lipoamide-dependent NADH oxidation Glycine max NAD+ + lipoamide
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.6 6.2
-
 ferrileghemoglobin recombinant enzyme Glycine max
1.6.2.6 6.2
-
 ferrileghemoglobin native enzyme Glycine max
1.6.2.6 31
-
 Lipoamide recombinant enzyme Glycine max