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Literature summary extracted from

  • Bellissimo, D.B.; Privalle, L.S.
    Expression of spinach nitrite reductase in Escherichia coli: site-directed mutagenesis of predicted active site amino acids (1995), Arch. Biochem. Biophys., 323, 155-163.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.7.1 fusion with beta-galactosidase Spinacia oleracea

Protein Variants

EC Number Protein Variants Comment Organism
1.7.7.1 C514S almost complete loss of activity Spinacia oleracea
1.7.7.1 C518S almost complete loss of activity Spinacia oleracea
1.7.7.1 G513A increased Km for nitrite compared to the wild type enzyme Spinacia oleracea
1.7.7.1 G513E loss of most of the activity Spinacia oleracea
1.7.7.1 G513V loss of most of the activity Spinacia oleracea
1.7.7.1 G519A 68% activity of wild type enzyme Spinacia oleracea
1.7.7.1 G519E 8% activity of wild type enzyme Spinacia oleracea
1.7.7.1 G519V 9% activity of wild type enzyme Spinacia oleracea
1.7.7.1 P515A marginal loss of activity Spinacia oleracea
1.7.7.1 P515S marginal loss of activity Spinacia oleracea
1.7.7.1 P515T marginal loss of activity Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.1 0.3
-
 nitrite
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.1 61000
-
 SDS-PAGE Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.1 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.1 homogeneity Spinacia oleracea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.1 100
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.1 nitrite + reduced ferredoxin
-
 Spinacia oleracea ammonia + oxidized ferredoxin
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.7.1 siroheme
-
 Spinacia oleracea